CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art
instrumentation or the need for years of hands-on experience, for better or worse, ensures …

A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

Identification of host genes essential for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding …

Genetic interactions mediate the emergence of phenotype from genotype, but technologies
for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we …

The genetic circuits that allow cancer cells to evade destruction by the host immune system
remain poorly understood,–. Here, to identify a phenotypically robust core set of genes and …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Diffuse large B cell lymphoma (DLBCL) is the most common form of blood cancer and is
characterized by a striking degree of genetic and clinical heterogeneity. This heterogeneity …

Understanding the functional consequences of single-nucleotide variants is critical to
uncovering the genetic underpinnings of diseases, but technologies to characterize variants …