In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Optical imaging has been essential for scientific observations to date, however its
biomedical applications has been restricted due to its poor penetration through tissues. In …

A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …

The last decade has seen the development of a wide set of tools, such as wavefront
sha**, computational or fundamental methods, that allow us to understand and control …

High-resolution optical imaging deep in tissues is challenging because of optical
aberrations and scattering of light caused by the complex structure of living matter. Here we …

Multiphoton fluorescence microscopy (MPFM) has been a game-changer for optical
imaging, particularly for studying biological tissues deep within living organisms. MPFM …

The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

Optical microscopy is widely used to visualize fine structures. When applied to bioimaging,
its performance is often degraded by sample-induced aberrations. In recent years, adaptive …

Deep tissue chemical imaging has a vital role in biological and medical applications.
Current approaches suffer from water absorption and tissue scattering, which limits imaging …

Understanding complex biological systems requires visualizing structures and processes
deep within living organisms. We developed a compact adaptive optics module and …