In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality
solution and solid-state NMR spectra of biological samples, with emphasis on integral …

The environment and unique balance of molecular forces within lipid bilayers has a
profound impact upon the structure, dynamics, and function of membrane proteins. We …

Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-
resolution analysis of the structure and topology of transmembrane proteins in native-like …

Oriented solid-state NMR is the most direct methodology to obtain the orientation of
membrane proteins with respect to the lipid bilayer. The method consists of measuring 1 H …

The anisotropic spin interactions measured for membrane proteins in weakly oriented
micelles and in oriented lipid bilayers provide independent and potentially complementary …

The β-barrels found in the outer membranes of prokaryotic and eukaryotic organisms
constitute an important functional class of proteins. Here we present solid-state NMR spectra …

AssignFit is a computer program developed within the XPLOR-NIH package for the
assignment of dipolar coupling (DC) and chemical shift anisotropy (CSA) restraints derived …

NMR spectroscopy enables the structures of membrane proteins to be determined in the
native-like environment of the phospholipid bilayer membrane. This chapter outlines the …

The classical Frenet frame is a moving frame on a smooth curve. Connecting a sequence of
points in space by line segments makes a discrete curve. The reference frame consisting of …