[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …
bioproduction. These properties can be introduced using various enzyme engineering …
[HTML][HTML] Optimizing microbial networks through metabolic bypasses
Metabolism has long been considered as a relatively stiff set of biochemical reactions. This
somewhat outdated and dogmatic view has been challenged over the last years, as multiple …
somewhat outdated and dogmatic view has been challenged over the last years, as multiple …
In Vitro Inhibitory Activity and Molecular Docking Study of Selected Natural Phenolic Compounds as AR and SDH Inhibitors**
Polyol pathway enzymes, aldose reductase (EC 1.1. 1.21; AR, ALR2), and sorbitol
dehydrogenase (EC 1.1. 1.14; SDH, SORD) have been widely investigated as the enzymes …
dehydrogenase (EC 1.1. 1.14; SDH, SORD) have been widely investigated as the enzymes …
[HTML][HTML] Harnessing noncanonical redox cofactors to advance synthetic assimilation of one-carbon feedstocks
Highlights•We discuss the use of NCRCs for supporting one-carbon assimilation.•
Engineered one-carbon oxidizing enzymes can accept NCRCs.•NCRCs can lower the …
Engineered one-carbon oxidizing enzymes can accept NCRCs.•NCRCs can lower the …
Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase
Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN+)
provide enhanced scalability for biomanufacturing. However, engineering enzymes to …
provide enhanced scalability for biomanufacturing. However, engineering enzymes to …
Shifting redox reaction equilibria on demand using an orthogonal redox cofactor
Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD+) and nicotinamide
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …
Design, construction, and application of noncanonical redox cofactor infrastructures
WB Black, S Perea, H Li - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•Noncanonical redox cofactors (NRCs) can overcome the limitations of natural
cofactors.•NRCs enable specific delivery of reducing power outside of biological …
cofactors.•NRCs enable specific delivery of reducing power outside of biological …
Cutting-edge computational approaches in enzyme design and activity enhancement
R Sun, D Wu, P Chen, P Zheng - Biochemical Engineering Journal, 2024 - Elsevier
Enzyme activity is crucial in biocatalysis, making methods to enhance enzyme performance
a major focus of research. Computational design provides an efficient approach to boosting …
a major focus of research. Computational design provides an efficient approach to boosting …
Computer-Aided Engineering of a Non-Phosphorylating Glyceraldehyde-3-Phosphate Dehydrogenase to Enable Cell-Free Biocatalysis
Redox cofactor utilization is one of the major barriers to the realization of efficient and cost-
competitive cell-free biocatalysis, especially where multiple redox steps are concerned. The …
competitive cell-free biocatalysis, especially where multiple redox steps are concerned. The …
Engineering Escherichia coli Pyruvate Metabolism to Generate Noncanonical Reducing Power
The future of biomanufacturing is dependent on rewiring biological systems to establish an
alternative approach to our current chemical industries. However, a key limitation in …
alternative approach to our current chemical industries. However, a key limitation in …