The de novo synthesis of genomes has made unprecedented progress and achieved
milestones, particularly in bacteria and yeast. However, the process of synthesizing a …

Synthetic genomics seeks to design and construct entire genomes to mechanistically dissect
fundamental questions of genome function and to engineer organisms for diverse …

The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

Pioneering advances in genome engineering, and specifically in genome writing, have
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …

We describe the complete synthesis, assembly, debugging, and characterization of a
synthetic 404,963 bp chromosome, synIX (synthetic chromosome IX). Combined …

De novo chromosome synthesis is costly and time-consuming, limiting its use in research
and biotechnology. Building synthetic chromosomes from natural components is an …

Regulation of eukaryotic gene expression involves a dynamic interplay between the core
transcriptional machinery, transcription factors, and chromatin organization and modification …

The Sc2. 0 global consortium to design and construct a synthetic genome based on the
Saccharomyces cerevisiae genome commenced in 2006, comprising 16 synthetic …

Pre‐mRNA splicing, the removal of introns and ligation of flanking exons, is a crucial step in
eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five …

Chromosome engineering is a transformative field at the cutting edge of biological science,
offering unprecedented precision in manipulating large-scale genomic DNA within cells …