Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues

KR Hall, KJ Robins, EM Williams, MH Rich, MJ Calcott… - Elife, 2020 - elifesciences.org
Selection for a promiscuous enzyme activity provides substantial opportunity for competition
between endogenous and newly-encountered substrates to influence the evolutionary …

Characterisation and optimisation of nitroreductase-prodrug combinations for bacterial-directed enzyme-prodrug therapy

J Chan-Hyams - 2020 - openaccess.wgtn.ac.nz
Gene-directed enzyme-prodrug therapy (GDEPT) employs tumour-tropic vectors including
viruses (VDEPT) and bacteria (BDEPT) to deliver a genetically-encoded prodrug-converting …

A giant leap in sequence space reveals the intracellular complexities of evolving a new function

KR Hall, KJ Robins, MH Rich, MJ Calcott, JN Copp… - BioRxiv, 2020 - biorxiv.org
Selection for a promiscuous enzyme activity provides substantial opportunity for competition
between endogenous and new substrates to influence the evolutionary trajectory, an aspect …