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Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Metabolic engineering: methodologies and applications
Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …
through the genetic manipulation of microbial metabolism. While the discipline is a little over …
Metabolic engineering of Escherichia coli for natural product biosynthesis
Natural products are widely employed in our daily lives as food additives, pharmaceuticals,
nutraceuticals, and cosmetic ingredients, among others. However, their supply has often …
nutraceuticals, and cosmetic ingredients, among others. However, their supply has often …
Engineering cellular metabolism
Metabolic engineering is the science of rewiring the metabolism of cells to enhance
production of native metabolites or to endow cells with the ability to produce new products …
production of native metabolites or to endow cells with the ability to produce new products …
[HTML][HTML] Bacteria as an alternate biofactory for carotenoid production: A review of its applications, opportunities and challenges
Carotenoid has gained a reputation amongst researchers for its robust antioxidant
capabilities. This review reasons the inevitability of carotenoid for a larger role than just …
capabilities. This review reasons the inevitability of carotenoid for a larger role than just …
Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …
The evolution of polystyrene as a cell culture material
Polystyrene (PS) has brought in vitro cell culture from its humble beginnings to the modern
era, propelling dozens of research fields along the way. This review discusses the …
era, propelling dozens of research fields along the way. This review discusses the …
Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
Recent developments in synthetic biology, combined with continued progress in systems
biology and metabolic engineering, have enabled the engineering of microorganisms to …
biology and metabolic engineering, have enabled the engineering of microorganisms to …
Microbial engineering for the production of advanced biofuels
Advanced biofuels produced by microorganisms have similar properties to petroleum-based
fuels, and can'drop in'to the existing transportation infrastructure. However, producing these …
fuels, and can'drop in'to the existing transportation infrastructure. However, producing these …
Precise and reliable gene expression via standard transcription and translation initiation elements
An inability to reliably predict quantitative behaviors for novel combinations of genetic
elements limits the rational engineering of biological systems. We developed an expression …
elements limits the rational engineering of biological systems. We developed an expression …