Protein glycosylation involves the co-translational or post-translational addition of glycans to
proteins and is a crucial protein modification in health and disease. The aim of …

Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides
(glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate …

Here, we review the current state of the art in analytical and structural methods used to
characterize therapeutic monoclonal antibodies (mAbs) and derivatives. First, a general …

Over the past few years, loss of patent protection for blockbuster monoclonal antibody (mAb)
drugs has caused a significant shift in the pharmaceutical industry towards the development …

In the last years, advances in ultra-high performance liquid chromatography (UHPLC)
coupled to mass spectrometry (MS) have been increased exponentially. This technique has …

Proteins are increasingly used as therapeutics. Their characterization is challenging due to
their size and inherent heterogeneity notably caused by post-translational modifications …

Human glycoproteins exhibit enormous heterogeneity at each N-glycosite, but few studies
have attempted to globally characterize the site-specific structural features. We have …

Glycosylation is one of the most significant and abundant post-translational modifications in
cells. Glycomic and glycoproteomic analyses involve the characterization of …

The sequence-specific retention calculator algorithm (SSRCalc)[Krokhin, OV Anal. Chem.
2006, 78, 7785] was adapted for the prediction of retention times of N-glycopeptides …

Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is
critical for drug development but remains challenging due to the inherent structural …