▪ Abstract Intein is the protein equivalent of intron and has been discovered in increasing
numbers of organisms and host proteins. A self-splicing intein catalyzes its own removal …

Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this
process known as protein splicing, the intein itself is not present in the final sequence, thus …

While the role of group I and group II intron‐encoded proteins in homing has been well
defined, the function of these proteins in intron dissemination to new sites remains the …

The homing endonuclease I‐Cre I recognizes and cleaves a particular 22 bp DNA
sequence. The crystal structure of I‐Cre I bound to homing site DNA has previously been …

RecA and Rad51 proteins are essential for homologous recombination in Bacteria
andEukarya, respectively. Homologous proteins, called RadA, have been described for …

Inteins possess two different enzymatic activities, self-catalyzed protein splicing and site-
specific DNA cleavage. These endonucleases, which are classified as part of the homing …

Mobile genetic elements constitute a remarkably diverse group of nonessential selfish
genes that provide no apparent function to the host. These selfish genes have been …

Single-stranded DNA-binding protein in Bacteria and replication protein A (RPA) in Eukarya
play crucial roles in DNA replication, repair, and recombination processes. We identified an …

In the evolution of life, DNA replication is a fundamental process, by which species transfer
their genetic information to their offspring. DNA polymerases, including bacterial and …

To search for restriction endonucleases, we used a novel plant-based cell-free translation
procedure that bypasses the toxicity of these enzymes. To identify candidate genes, the …