The initiation of eukaryotic DNA replication
DNA replication in eukaryotic cells initiates from large numbers of sites called replication
origins. Initiation of replication from these origins must be tightly controlled to ensure the …
origins. Initiation of replication from these origins must be tightly controlled to ensure the …
The plasticity of DNA replication forks in response to clinically relevant genotoxic stress
Complete and accurate DNA replication requires the progression of replication forks through
DNA damage, actively transcribed regions, structured DNA and compact chromatin. Recent …
DNA damage, actively transcribed regions, structured DNA and compact chromatin. Recent …
Fast and efficient DNA replication with purified human proteins
Chromosome replication is performed by a complex and intricate ensemble of proteins
termed the replisome, where the DNA polymerases Polδ and Polε, DNA polymerase α …
termed the replisome, where the DNA polymerases Polδ and Polε, DNA polymerase α …
The structural mechanism of dimeric DONSON in replicative helicase activation
MA Cvetkovic, P Passaretti, A Butryn… - Molecular Cell, 2023 - cell.com
The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …
Mechanism of replication origin melting nucleated by CMG helicase assembly
The activation of eukaryotic origins of replication occurs in temporally separated steps to
ensure that chromosomes are copied only once per cell cycle. First, the MCM helicase is …
ensure that chromosomes are copied only once per cell cycle. First, the MCM helicase is …
Structure of a human replisome shows the organisation and interactions of a DNA replication machine
The human replisome is an elaborate arrangement of molecular machines responsible for
accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG) helicase …
accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG) helicase …
Cryo-EM structure of the fork protection complex bound to CMG at a replication fork
The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase,
orchestrates chromosome replication. Multiple factors associate directly with CMG, including …
orchestrates chromosome replication. Multiple factors associate directly with CMG, including …
[HTML][HTML] A conserved mechanism for regulating replisome disassembly in eukaryotes
Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by
ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase,–. Despite being driven …
ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase,–. Despite being driven …
Structure of the processive human Pol δ holoenzyme
In eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen
(PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to …
(PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to …
Single-molecule imaging reveals replication fork coupled formation of G-quadruplex structures hinders local replication stress signaling
Guanine-rich DNA sequences occur throughout the human genome and can transiently form
G-quadruplex (G4) structures that may obstruct DNA replication, leading to genomic …
G-quadruplex (G4) structures that may obstruct DNA replication, leading to genomic …