The sporophyte-to-gametophyte transition: The haploid generation comes of age

J Somers, B Nelms - Current Opinion in Plant Biology, 2023 - Elsevier
Flowering plants alternate between two multicellular generations: the diploid sporophyte
and haploid gametophyte. Despite its small size, the gametophyte has significant impacts on …

Evolutionary genomics of plant gametophytic selection

FEG Beaudry, JL Rifkin, SCH Barrett, SI Wright - Plant Communications, 2020 - cell.com
It has long been recognized that natural selection during the haploid gametophytic phase of
the plant life cycle may have widespread importance for rates of evolution and the …

The maize gene maternal derepression of r1 encodes a DNA glycosylase that demethylates DNA and reduces siRNA expression in the endosperm

JI Gent, KM Higgins, KW Swentowsky, FF Fu… - The Plant …, 2022 - academic.oup.com
Demethylation of transposons can activate the expression of nearby genes and cause
imprinted gene expression in the endosperm; this demethylation is hypothesized to lead to …

Gametophyte genome activation occurs at pollen mitosis I in maize

B Nelms, V Walbot - Science, 2022 - science.org
Flowering plants alternate between multicellular haploid (gametophyte) and diploid
(sporophyte) generations. Pollen actively transcribes its haploid genome, providing …

Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes

X Li, A Bruckmann, T Dresselhaus, K Begcy - Plant Physiology, 2024 - academic.oup.com
For successful double fertilization in flowering plants (angiosperms), pollen tubes deliver 2
nonmotile sperm cells toward female gametes (egg and central cell, respectively) …

Long-read cDNA sequencing enables a “gene-like” transcript annotation of transposable elements

K Panda, RK Slotkin - Plant Cell, 2020 - academic.oup.com
Transcript-based annotations of genes facilitate both genome-wide analyses and detailed
single-locus research. In contrast, transposable element (TE) annotations are rudimentary …

Potent pollen gene regulation by DNA glycosylases in maize

Y Zeng, J Somers, HS Bell, Z Vejlupkova… - Nature …, 2024 - nature.com
Although DNA methylation primarily represses TEs, it also represses select genes that are
methylated in plant body tissues but demethylated by DNA glycosylases (DNGs) in …

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

EM Khalaf, A Shrestha, M Reid, BJ McFadyen… - Frontiers in …, 2023 - frontiersin.org
Pollen is a vector for diversification, fitness-selection, and transmission of plant genetic
material. The extent to which the pollen microbiome may contribute to host diversification is …

qTeller: A tool for comparative multi-genomic gene expression analysis

MR Woodhouse, S Sen, D Schott, JL Portwood… - …, 2022 - academic.oup.com
Motivation Over the last decade, RNA-Seq whole-genome sequencing has become a widely
used method for measuring and understanding transcriptome-level changes in gene …

EASTR: Identifying and eliminating systematic alignment errors in multi-exon genes

I Shinder, R Hu, HJ Ji, KH Chao, M Pertea - Nature communications, 2023 - nature.com
Accurate alignment of transcribed RNA to reference genomes is a critical step in the analysis
of gene expression, which in turn has broad applications in biomedical research and in the …