Challenges and best practices in omics benchmarking

TG Brooks, NF Lahens, A Mrčela, GR Grant - Nature Reviews Genetics, 2024 - nature.com
Technological advances enabling massively parallel measurement of biological features—
such as microarrays, high-throughput sequencing and mass spectrometry—have ushered in …

[HTML][HTML] Leveraging multi-omics data to infer regulators of mRNA 3'end processing in glioblastoma

A Mironov, L Franchitti, S Ghosh, MF Ritz… - Frontiers in Molecular …, 2024 - frontiersin.org
Alterations in mRNA 3'end processing and polyadenylation are widely implicated in the
biology of many cancer types, including glioblastoma (GBM), one the most aggressive tumor …

Alternative Transcripts Diversify Genome Function for Phenome Relevance to Health and Diseases

SA Carrion, JJ Michal, Z Jiang - Genes, 2023 - mdpi.com
Manipulation using alternative exon splicing (AES), alternative transcription start (ATS), and
alternative polyadenylation (APA) sites are key to transcript diversity underlying health and …

PolyASite v3. 0: a multi-species atlas of polyadenylation sites inferred from single-cell RNA-sequencing data

Y Moon, CJ Herrmann, A Mironov… - Nucleic Acids …, 2025 - academic.oup.com
The broadly used 10X Genomics technology for single-cell RNA sequencing (scRNA-seq)
captures RNA 3′ ends. Thus, some reads contain part of the non-templated polyadenosine …

[HTML][HTML] TDP-43 loss induces extensive cryptic polyadenylation in ALS/FTD

S Bryce-Smith, AL Brown, PR Mehta, F Mattedi… - BioRxiv, 2024 - ncbi.nlm.nih.gov
Nuclear depletion and cytoplasmic aggregation of the RNA-binding protein TDP-43 is the
hallmark of ALS, occurring in over 97% of cases. A key consequence of TDP-43 nuclear loss …

Alternative 3′ UTR expression induced by T cell activation is regulated in a temporal and signal dependent manner

D Blake, MR Gazzara, I Breuer, M Ferretti… - Scientific Reports, 2024 - nature.com
The length of 3′ untranslated regions (3′ UTR) is highly regulated during many transitions
in cell state, including T cell activation, through the process of alternative polyadenylation …

TDP-43 dysregulation of polyadenylation site selection is a defining feature of RNA misprocessing in ALS/FTD and related disorders

FJ Arnold, Y Cui, S Michels, MR Colwin, C Stockford… - BioRxiv, 2024 - biorxiv.org
Nuclear clearance and cytoplasmic aggregation of the RNA-binding protein TDP-43 are
observed in many neurodegenerative disorders, including amyotrophic lateral sclerosis …

ZARP: A user-friendly and versatile RNA-seq analysis workflow

M Katsantoni, F Gypas, CJ Herrmann, D Burri… - …, 2024 - f1000research.com
Background RNA sequencing (RNA-seq) is a widely used technique in many scientific
studies. Given the plethora of models and software packages that have been developed for …

[HTML][HTML] InPAS: An R/Bioconductor Package for Identifying Novel Polyadenylation Sites and Alternative Polyadenylation from Bulk RNA-seq Data

J Ou, H Liu, S Park, MR Green, LJ Zhu - Frontiers in Bioscience …, 2024 - imrpress.com
Background: Alternative cleavage and polyadenylation (APA) is a crucial post-transcriptional
gene regulation mechanism that regulates gene expression in eukaryotes by increasing the …

Deep Learning Models of Polyadenylation Regulation and Applications in Genetics

EK Stroup - 2024 - search.proquest.com
RNA processing tightly couples transcription to the 5'cap**, intron splicing, and 3'-end
formation of pre-mRNA to produce a mature mRNA prepared for export to the cytoplasm and …