The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such
as expression of recombinant proteins, requires media that support growth and production …

The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …

Nearly 80% of the approved human therapeutic antibodies are produced by Chinese
Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant …

Large scale biopharmaceutical production of biologics relies on the overexpression of
foreign proteins by cells cultivated in stirred tank bioreactors. It is well recognized and …

Amino acids are crucial for the cultivation of mammalian cells. This importance of amino
acids was realized soon after the development of the first cell lines, and a solution of a …

Chinese hamster ovary (CHO) cells are the most widely used mammalian host cells for the
commercial production of therapeutic proteins. Fed-batch culture is widely used to produce …

Organic waste is a valuable raw material with potential for replacing synthetic products of
petroleum origin in a more sustainable economy. Such a material is unmarketable wool …

Cysteine is considered an essential amino acid in the cultivation of Chinese hamster ovary
(CHO) cells. An optimized cysteine supply during fed‐batch cultivation supports the protein …

There is continual demand to maximize CHO cell culture productivity of a biotherapeutic
while maintaining product quality. In this study, a comprehensive multi‐omics analysis is …

Cysteine and cystine are essential amino acids present in mammalian cell cultures. While
contributing to biomass synthesis, recombinant protein production, and antioxidant defense …