Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …

In polycystic kidney disease (PKD), microscopic tubules expand into macroscopic cysts.
Among the world's most common genetic disorders, PKD is inherited via heterozygous loss …

Base editors (BEs) enable efficient, programmable installation of point mutations while
avoiding the use of double-strand breaks. Simultaneous application of two or more different …

Long QT syndrome (LQTS) is a cardiovascular disease characterized by QT interval
prolongation that can lead to sudden cardiac death. Many mutations with heterogeneous …

Prime editing is a versatile genome-editing technique that shows great promise for the
generation and repair of patient mutations. However, some genomic sites are difficult to edit …

Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that
allows the introduction of short insertions, deletions, and substitutions into the genome …

Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA
engineering and genome editing tools. However, in many primary cell types, the efficiency of …

Human pluripotent stem cells (hPSCs) are uniquely suited to study human development and
disease and promise to revolutionize regenerative medicine. These applications rely on …

Human population is expected to reach to about 10 billion by 2050. Climate change affects
crop production, thus posing food security challenges. Conventional breeding alone will not …