Co-receptor signaling in the pathogenesis of neuroHIV
The HIV co-receptors, CCR5 and CXCR4, are necessary for HIV entry into target cells,
interacting with the HIV envelope protein, gp120, to initiate several signaling cascades …
interacting with the HIV envelope protein, gp120, to initiate several signaling cascades …
Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain
Frizzleds (FZD1–10) are G protein–coupled receptors containing an extracellular cysteine-
rich domain (CRD) binding Wingless/Int-1 lipoglycoproteins (WNTs). Despite the role of …
rich domain (CRD) binding Wingless/Int-1 lipoglycoproteins (WNTs). Despite the role of …
Microscopy and spectroscopy approaches to study GPCR structure and function
The GPCR signalling cascade is a key pathway responsible for the signal transduction of a
multitude of physical and chemical stimuli, including light, odorants, neurotransmitters and …
multitude of physical and chemical stimuli, including light, odorants, neurotransmitters and …
Itch receptor MRGPRX4 interacts with the receptor activity–modifying proteins
IB Kotliar, E Ceraudo, K Kemelmakher-Liben… - Journal of Biological …, 2023 - ASBMB
Cholestatic itch is a severe and debilitating symptom in liver diseases with limited treatment
options. The class AG protein-coupled receptor (GPCR) Mas-related GPCR subtype X4 …
options. The class AG protein-coupled receptor (GPCR) Mas-related GPCR subtype X4 …
Competitive binding study revealing the influence of fluorophore labels on biomolecular interactions
MS Dietz, SS Wehrheim, MLIE Harwardt… - Nano …, 2019 - ACS Publications
Fluorescence methods are important tools in modern biology. Direct labeling of
biomolecules with a fluorophore might, however, change interaction surfaces. Here, we …
biomolecules with a fluorophore might, however, change interaction surfaces. Here, we …
The use of fluorescence correlation spectroscopy to characterise the molecular mobility of G protein-coupled receptors in membrane microdomains: an update
It has become increasingly apparent that some G protein-coupled receptors (GPCRs) are
not homogeneously expressed within the plasma membrane but may instead be organised …
not homogeneously expressed within the plasma membrane but may instead be organised …
[HTML][HTML] Advances in the application of fluorescence correlation spectroscopy to study detergent purified and encapsulated membrane proteins
LA Stoddart, J Goulding, SJ Briddon - The International Journal of …, 2022 - Elsevier
Fluorescence correlation spectroscopy (FCS) is a quantitative spectroscopy technique which
could potentially increase throughput and sensitivity of screening for ligand, substrate and …
could potentially increase throughput and sensitivity of screening for ligand, substrate and …
Preparation of a stable CCL5· CCR5· Gi signaling complex for Cryo-EM analysis
The numerous chemokines and their cognate G protein-coupled chemokine receptors on
the surface of leukocytes form a complex signaling network, which regulates the immune …
the surface of leukocytes form a complex signaling network, which regulates the immune …
[HTML][HTML] On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies
Chemokine receptors, a subfamily of G-protein-coupled receptors (GPCRs), are responsible
for cell migration during physiological processes as well as in diseases like inflammation …
for cell migration during physiological processes as well as in diseases like inflammation …
Efficient production of fluorophore‐labeled CC chemokines for biophysical studies using recombinant enterokinase and recombinant sortase
Chemokines are important immune system proteins, many of which mediate inflammation
due to their function to activate and cause chemotaxis of leukocytes. An important anti …
due to their function to activate and cause chemotaxis of leukocytes. An important anti …