Filamentous fungi drive carbon and nutrient cycling across our global ecosystems, through
its interactions with growing and decaying flora and their constituent microbiomes. The …

Background Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology
have depended on increasingly sophisticated vector platforms for analysis and engineering …

Split reporter proteins capable of self-association and reactivation have applications in
biomedical research, but designing these proteins, especially the selection of appropriate …

Protein translocation by the bacterial type VI secretion system (T6 SS) is driven by a rapid
contraction of a sheath assembled around a tube with associated effectors. Here, we show …

Abstract Domain recombination is a key principle in protein evolution and protein
engineering, but inserting a donor domain into every position of a target protein is not easily …

Plasmids are important mobile elements in bacteria, contributing to evolution, virulence, and
antibiotic resistance. Natural plasmids are generally large and maintained at low copy …

Peptide tags fused to proteins are used in a variety of applications, including as affinity tags
for purification, epitope tags for immunodetection, or fluorescent protein tags for …

Genome‐wide identification of transcription factor binding sites with the ChIP‐seq method is
an extremely important scientific endeavor− one that should ideally be performed for every …

Random transposon mutagenesis is a powerful and unbiased genetic approach to answer
fundamental biological questions. Here, we introduce an improved mariner-based …

A simple, broadly applicable method was developed using an in vitro transposition reaction
followed by transformation into Escherichia coli and screening plates for fluorescent …