RNA sequencing: the teenage years

R Stark, M Grzelak, J Hadfield - Nature Reviews Genetics, 2019‏ - nature.com
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

A field guide to whole‐genome sequencing, assembly and annotation

R Ekblom, JBW Wolf - Evolutionary applications, 2014‏ - Wiley Online Library
Genome sequencing projects were long confined to biomedical model organisms and
required the concerted effort of large consortia. Rapid progress in high‐throughput …

Corset: enabling differential gene expression analysis for de novoassembled transcriptomes

NM Davidson, A Oshlack - Genome biology, 2014‏ - Springer
Next generation sequencing has made it possible to perform differential gene expression
studies in non-model organisms. For these studies, the need for a reference genome is …

Bioinformatic processing of RAD‐seq data dramatically impacts downstream population genetic inference

ABA Shafer, CR Peart, S Tusso… - Methods in Ecology …, 2017‏ - Wiley Online Library
Restriction site‐associated DNA sequencing (RAD‐seq) provides high‐resolution
population genomic data at low cost, and has become an important component in ecological …

Mining and development of novel SSR markers using next generation sequencing (NGS) data in plants

S Taheri, T Lee Abdullah, MR Yusop, MM Hanafi… - Molecules, 2018‏ - mdpi.com
Microsatellites, or simple sequence repeats (SSRs), are one of the most informative and
multi-purpose genetic markers exploited in plant functional genomics. However, the …

The power and promise of RNA‐seq in ecology and evolution

EV Todd, MA Black, NJ Gemmell - Molecular ecology, 2016‏ - Wiley Online Library
Reference is regularly made to the power of new genomic sequencing approaches. Using
powerful technology, however, is not the same as having the necessary power to address a …

Atlas of ACE2 gene expression reveals novel insights into transmission of SARS-CoV-2

K Sun, L Gu, L Ma, Y Duan - Heliyon, 2021‏ - cell.com
Abstract The recent pandemic, COVID-19, is caused by a novel coronavirus, SARS-CoV-2,
with elusive origin. SARS-CoV-2 infects mammalian cells via ACE2, a transmembrane …

Recent progress and challenges in population genetics of polyploid organisms: an overview of current state‐of‐the‐art molecular and statistical tools

F Dufresne, M Stift, R Vergilino, BK Mable - Molecular ecology, 2014‏ - Wiley Online Library
Despite the importance of polyploidy and the increasing availability of new genomic data,
there remain important gaps in our knowledge of polyploid population genetics. These gaps …

Power analysis and sample size estimation for RNA-Seq differential expression

T Ching, S Huang, LX Garmire - Rna, 2014‏ - rnajournal.cshlp.org
It is crucial for researchers to optimize RNA-seq experimental designs for differential
expression detection. Currently, the field lacks general methods to estimate power and …

Introducing BASE: the Biomes of Australian Soil Environments soil microbial diversity database

A Bissett, A Fitzgerald, T Meintjes, PM Mele, F Reith… - GigaScience, 2016‏ - Springer
Background Microbial inhabitants of soils are important to ecosystem and planetary
functions, yet there are large gaps in our knowledge of their diversity and ecology. The …