Acquisition and analysis of DIA-based proteomic data: A comprehensive survey in 2023

R Lou, W Shui - Molecular & Cellular Proteomics, 2024 - ASBMB
Data-independent acquisition (DIA) mass spectrometry (MS) has emerged as a powerful
technology for high-throughput, accurate, and reproducible quantitative proteomics. This …

What can ribo-seq, immunopeptidomics, and proteomics tell us about the noncanonical proteome?

JR Prensner, JG Abelin, LW Kok, KR Clauser… - Molecular & Cellular …, 2023 - ASBMB
Abstract Ribosome profiling (Ribo-Seq) has proven transformative for our understanding of
the human genome and proteome by illuminating thousands of noncanonical sites of …

Thunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model

D Gomez-Zepeda, D Arnold-Schild, J Beyrle… - Nature …, 2024 - nature.com
Human leukocyte antigen (HLA) class I peptide ligands (HLAIps) are key targets for
develo** vaccines and immunotherapies against infectious pathogens or cancer cells …

[HTML][HTML] Rescoring Peptide Spectrum Matches: Boosting Proteomics Performance by Integrating Peptide Property Predictors into Peptide Identification

M Kalhor, J Lapin, M Picciani, M Wilhelm - Molecular & Cellular Proteomics, 2024 - Elsevier
Rescoring of peptide spectrum matches originating from database search engines enabled
by peptide property predictors is exceeding the performance of peptide identification from …

OpenProt 2.0 builds a path to the functional characterization of alternative proteins

S Leblanc, F Yala, N Provencher, JF Lucier… - Nucleic Acids …, 2024 - academic.oup.com
The OpenProt proteogenomic resource (https://www. openprot. org/) provides users with a
complete and freely accessible set of non-canonical or alternative open reading frames …

The Proteomics Standards Initiative standardized formats for spectral libraries and fragment ion peak annotations: mzSpecLib and mzPAF

J Klein, H Lam, TD Mak, W Bittremieux… - Analytical …, 2024 - ACS Publications
Mass spectral libraries are collections of reference spectra, usually associated with specific
analytes from which the spectra were generated, that are used for further downstream …

ProHap enables human proteomic database generation accounting for population diversity

J Vašíček, KG Kuznetsova, D Skiadopoulou, L Unger… - Nature …, 2024 - nature.com
Amid the advances in genomics, the availability of large reference panels of human
haplotypes is key to account for human diversity within and across populations. However …

Koina: Democratizing machine learning for proteomics research

L Lautenbacher, KL Yang, T Kockmann, C Panse… - …, 2024 - pmc.ncbi.nlm.nih.gov
Recent developments in machine-learning (ML) and deep-learning (DL) have immense
potential for applications in proteomics, such as generating spectral libraries, improving …

ADAM19 cleaves the PTH receptor and associates with brachydactyly type E

A Aydin, C Klenk, K Nemec, A Işbilir… - Life Science …, 2024 - life-science-alliance.org
Brachydactyly type E (BDE), shortened metacarpals, metatarsals, cone-shaped epiphyses,
and short stature commonly occurs as a sole phenotype. Parathyroid hormone-like protein …

Proteogenomics analysis of human tissues using pangenomes

D Wang, R Bouwmeester, P Zheng, C Dai, A Sanchez… - bioRxiv, 2024 - biorxiv.org
The genomics landscape is evolving with the emergence of pangenomes, challenging the
conventional single-reference genome model. The new human pangenome reference …