Heat-induced antigen retrieval: mechanisms and application to histochemistry

S Yamashita - Progress in histochemistry and cytochemistry, 2007‏ - Elsevier
Since the introduction of the fluorescence-labeled antibody method by Coons et
al.[Immunological properties of antibody containing a fluorescent group. Proc Soc Exp Biol …

Structure and binding of unconjugated bilirubin: relevance for physiological and pathophysiological function

JD Ostrow, P Mukerjee, C Tiribelli - Journal of lipid research, 1994‏ - Elsevier
There is still much uncertainty concerning the structure, physical chemistry, transport, and
metabolism of unconjugated bilirubin (UCB), in both physiological and pathological states …

Functionalization of silk by AIEgens through facile bioconjugation: full‐color fluorescence and long‐term bioimaging

C Liu, H Bai, B He, X He, J Zhang… - Angewandte Chemie …, 2021‏ - Wiley Online Library
Silkworm silk is a promising natural biopolymer for textile and biomedical applications for its
remarkable flexibility, excellent biocompatibility and controllable biodegradability. The …

A dynamic model for bilirubin binding to human serum albumin

CE Petersen, CE Ha, K Harohalli, JB Feix… - Journal of Biological …, 2000‏ - jbc.org
Site-directed mutagenesis of human serum albumin was used to study the role of various
amino acid residues in bilirubin binding. A comparison of thermodynamic, proteolytic, and x …

Bio-responsive delivery of tissue plasminogen activator for localized thrombolysis

S Absar, YM Kwon, F Ahsan - Journal of Controlled Release, 2014‏ - Elsevier
In this study, we have developed an albumin-camouflaged/thrombin-triggered delivery
system for site-specific delivery of tissue plasminogen activator (tPA). The camouflaged …

Mass spectrometry, structural analysis, and anti-inflammatory properties of photo-cross-linked human albumin hydrogels

S Sharifi, AA Saei, H Gharibi… - ACS applied bio …, 2022‏ - ACS Publications
Albumin-based hydrogels offer unique benefits such as biodegradability and high binding
affinity to various biomolecules, which make them suitable candidates for biomedical …

Mechanisms of heat-induced antigen retrieval: does pH or ionic strength of the solution play a role for refolding antigens?

K Emoto, S Yamashita, Y Okada - Journal of Histochemistry …, 2005‏ - journals.sagepub.com
We investigated the effects of pH and ionic strength of solutions used for antigen retrieval to
elucidate the mechanism of heat-induced antigen retrieval (HIAR) in immunohistochemistry …

Preparation and evaluation of BSA-based hydrosol nanoparticles cross-linked with genipin for oral administration of poorly water-soluble curcumin

N Shahgholian, G Rajabzadeh… - International journal of …, 2017‏ - Elsevier
One of the most interesting functions of albumin is the ability to interact with bioactive
compounds. This study describes preparation of protein-based nanoparticles (NPs) for the …

Use of fluorescence enhancement technique to study bilirubin–albumin interaction

H Athar, N Ahmad, S Tayyab, MA Qasim - International journal of biological …, 1999‏ - Elsevier
Bilirubin–albumin solution gave an emission spectrum in the wavelength range 500–600 nm
with emission maxima at 528 nm when excited at 487 nm. The magnitude of fluorescence …

Understanding the role of internal lysine residues of serum albumins in conformational stability and bilirubin binding

MM Khan, S Tayyab - Biochimica et Biophysica Acta (BBA)-Protein …, 2001‏ - Elsevier
The role of internal lysine residues of different serum albumins, viz. from human, rabbit, goat,
sheep and buffalo (HSA, RbSA, GSA, SSA and BuSA), in conformational stability and …