DNA damage checkpoint execution and the rules of its disengagement

CQX Yam, HH Lim, U Surana - Frontiers in Cell and Developmental …, 2022 - frontiersin.org
Chromosomes are susceptible to damage during their duplication and segregation or when
exposed to genotoxic stresses. Left uncorrected, these lesions can result in genomic …

Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clam** enabled by multi-subunit conformational changes

Y Yu, S Li, Z Ser, H Kuang, T Than, D Guan… - Proceedings of the …, 2022 - pnas.org
Structural maintenance of chromosomes (SMC) complexes are essential for chromatin
organization and functions throughout the cell cycle. The cohesin and condensin SMCs fold …

Instant Inhibition and Subsequent Self-Adaptation of Chlorella sp. Toward Free Ammonia Shock in Wastewater: Physiological and Genetic Responses

Z Chen, S Qiu, M Li, D Zhou, S Ge - Environmental Science & …, 2022 - ACS Publications
Free ammonia (FA) has been recently demonstrated as the primary stress factor
suppressing microalgal activities in high-ammonium wastewater. However, its inhibition …

The separation pin distinguishes the pro– and anti–recombinogenic functions of Saccharomyces cerevisiae Srs2

A Meir, VB Raina, CE Rivera, L Marie… - Nature …, 2023 - nature.com
Srs2 is an Sf1a helicase that helps maintain genome stability in Saccharomyces cerevisiae
through its ability to regulate homologous recombination. Srs2 downregulates HR by …

Rtt105 regulates RPA function by configurationally stapling the flexible domains

S Kuppa, J Deveryshetty, R Chadda, JR Mattice… - Nature …, 2022 - nature.com
Abstract Replication Protein A (RPA) is a heterotrimeric complex that binds to single-
stranded DNA (ssDNA) and recruits over three dozen RPA-interacting proteins to coordinate …

Human HELB is a processive motor protein that catalyzes RPA clearance from single-stranded DNA

S Hormeno, OJ Wilkinson, C Aicart-Ramos… - Proceedings of the …, 2022 - pnas.org
Human DNA helicase B (HELB) is a poorly characterized helicase suggested to play both
positive and negative regulatory roles in DNA replication and recombination. In this work, we …

A DNA damage–induced phosphorylation circuit enhances Mec1ATR Ddc2ATRIP recruitment to Replication Protein A

LA Yates, EA Tannous, RM Morgan… - Proceedings of the …, 2023 - pnas.org
The cell cycle checkpoint kinase Mec1ATR and its integral partner Ddc2ATRIP are vital for
the DNA damage and replication stress response. Mec1–Ddc2 “senses” single-stranded …

Mms22-Rtt107 axis attenuates the DNA damage checkpoint and the stability of the Rad9 checkpoint mediator

B Wan, D Guan, S Li, T Chwat-Edelstein… - Nature …, 2025 - nature.com
The DNA damage checkpoint is a highly conserved signaling pathway induced by genotoxin
exposure or endogenous genome stress. It alters many cellular processes such as arresting …

Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments

A Meir, JB Crickard, Y Kwon, P Sung… - Proceedings of the …, 2022 - pnas.org
Srs2 is a superfamily 1 (SF1) helicase that participates in several pathways necessary for
the repair of damaged DNA. Srs2 regulates formation of early homologous recombination …

Replication stress response in fission yeast differentially depends on maintaining proper levels of Srs2 helicase and Rrp1, Rrp2 DNA translocases

G Baranowska, D Misiorna, W Białek, K Kramarz… - Plos one, 2024 - journals.plos.org
Homologous recombination is a key process that governs the stability of eukaryotic
genomes during DNA replication and repair. Multiple auxiliary factors regulate the choice of …