Sorting out the complexity of SR protein functions

BR Graveley - Rna, 2000 - cambridge.org
Members of the serine/arginine-rich (SR) protein family have multiple functions in the pre-
mRNA splicing reaction. In addition to being required for the removal of constitutively spliced …

Role of RNA structure in regulating pre-mRNA splicing

MB Warf, JA Berglund - Trends in biochemical sciences, 2010 - cell.com
Pre-mRNA splicing involves removing non-coding introns from RNA transcripts. It is carried
out by the spliceosome, along with other auxiliary factors. In general, research in splicing …

Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells

L Nissim, SD Perli, A Fridkin, P Perez-Pinera, TK Lu - Molecular cell, 2014 - cell.com
RNA-based regulation and CRISPR/Cas transcription factors (CRISPR-TFs) have the
potential to be integrated for the tunable modulation of gene networks. A major limitation of …

An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels.

B Rigat, C Hubert, F Alhenc-Gelas… - The Journal of …, 1990 - Am Soc Clin Investig
A polymorphism consisting of the presence or absence of a 250-bp DNA fragment was
detected within the angiotensin I-converting enzyme gene (ACE) using the endothelial ACE …

Prediction of human mRNA donor and acceptor sites from the DNA sequence

S Brunak, J Engelbrecht, S Knudsen - Journal of molecular biology, 1991 - Elsevier
Artificial neural networks have been applied to the prediction of splice site location in human
pre-mRNA. A joint prediction scheme where prediction of transition regions between introns …

ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genes

C Troelstra, A van Gool, J de Wit, W Vermeulen… - Cell, 1992 - cell.com
Cells from patients with the UV-sensitive nucleotide excision repair disorder Cockayne's
syndrome (CS) have a specific defect in preferential repair of lesions from the transcribed …

Genome-wide discovery of human splicing branchpoints

TR Mercer, MB Clark, SB Andersen, ME Brunck… - Genome …, 2015 - genome.cshlp.org
During the splicing reaction, the 5′ intron end is joined to the branchpoint nucleotide,
selecting the next exon to incorporate into the mature RNA and forming an intron lariat …

[PDF][PDF] Splicing of precursors to mRNAs by the spliceosomes

CB Burge, T Tuschl, PA Sharp - Cold Spring Harbor Monograph Series, 1999 - Citeseer
The splicing of precursors to mRNAs occurs in two steps, both involving single
transesterification reactions (Fig. 1). The first step generates a 2-5 bond at the branch site …

Genetic basis of variable exon 9 skip** in cystic fibrosis transmembrane conductance regulator mRNA

CS Chu, BC Trapnell, S Curristin, GR Cutting… - Nature …, 1993 - nature.com
Variable in–frame skip** of exon 9 in cystic fibrosis transmembrane conductance
regulator (CFTR) mRNA transcripts (exon 9−) occurs in the respiratory epithelium. To …

Cloning and domain structure of the mammalian splicing factor U2AF

PD Zamore, JG Patton, MR Green - Nature, 1992 - nature.com
A complementary DNA clone encoding the large subunit of the essential mammalian
premessenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been …