Clustered regularly interspaced short palindromic repeats (CRISPR) system provides
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …

While largely neglected over decades during which adaptive immunity captured most of the
attention, innate immune mechanisms have now become central to our understanding of …

Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …

BACKGROUND Prokaryotes have evolved multiple systems to combat invaders such as
viruses and plasmids. Examples of such defense systems include receptor masking …

Gene therapy by expression constructs or down-regulation of certain genes has shown great
potential for the treatment of various diseases. The wide clinical application of nucleic acid …

Prokaryotes have evolved several defence mechanisms to protect themselves from viral
predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their …

In recent years, CRISPR (clustered regularly interspaced short palindromic repeat)/Cas
(CRISPR-associated) genome editing systems have become one of the most robust …

Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …

Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

BACKGROUND The arms race between prokaryotes and their perpetually evolving
predators has fueled the evolution of a defense arsenal. The so-called CRISPR-Cas …