Asymmetric flow field-flow fractionation in the field of nanomedicine
Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the
family of field-flow fractionations, indicated by a rapidly increasing number of publications. It …
family of field-flow fractionations, indicated by a rapidly increasing number of publications. It …
[HTML][HTML] Field-flow fractionation for molecular-interaction studies of labile and complex systems: A critical review
IK Ventouri, S Loeber, GW Somsen… - Analytica chimica …, 2022 - Elsevier
Asymmetrical flow field-flow fractionation (AF4) has attracted considerable attention as a
size-based separation technique, due to its mild separation conditions, broad working range …
size-based separation technique, due to its mild separation conditions, broad working range …
Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)
The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and
patient safety. Typically, the analysis is performed in the finished formulation to ensure that …
patient safety. Typically, the analysis is performed in the finished formulation to ensure that …
Separation and characterization of extracellular vesicles from human plasma by asymmetrical flow field-flow fractionation
B Wu, X Chen, J Wang, X Qing, Z Wang, X Ding… - Analytica Chimica …, 2020 - Elsevier
It is a big challenge to isolate extracellular vesicles (EVs) from human plasma because of
the contamination from high abundant lipoproteins, such as high density lipoprotein (HDL) …
the contamination from high abundant lipoproteins, such as high density lipoprotein (HDL) …
Dielectrophoresis-field flow fractionation for separation of particles: A critical review
Dielectrophoresis-field flow fractionation (DEP-FFF) has emerged as an efficient in-vitro, non-
invasive, and label-free mechanism to manipulate a variety of nano-and micro-scaled …
invasive, and label-free mechanism to manipulate a variety of nano-and micro-scaled …
Probing protein denaturation during size-exclusion chromatography using native mass spectrometry
IK Ventouri, DBA Malheiro, RLC Voeten, S Kok… - Analytical …, 2020 - ACS Publications
Size-exclusion chromatography employing aqueous mobile phases with volatile salts at
neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a …
neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a …
Analysis of proteins, protein complexes, and organellar proteomes using sheathless capillary zone electrophoresis-native mass spectrometry
Native mass spectrometry (MS) is a rapidly advancing field in the analysis of proteins,
protein complexes, and macromolecular species of various types. The majority of native MS …
protein complexes, and macromolecular species of various types. The majority of native MS …
Understanding the Physical Interactions in the FGF21/FGFR/β‐Klotho Complex: Structural Requirements and Implications in FGF21 Signaling
The endocrine fibroblast growth factor 21 (FGF21) requires both fibroblast growth factor
receptor (FGFR) and β‐Klotho for signaling. In this study, we sought to understand the inter …
receptor (FGFR) and β‐Klotho for signaling. In this study, we sought to understand the inter …
Surface-induced dissociation of ion mobility-separated noncovalent complexes in a quadrupole/time-of-flight mass spectrometer
A custom in-line surface-induced dissociation (SID) device has been incorporated into a
commercial ion mobility quadrupole/time-of-flight mass spectrometer in order to provide an …
commercial ion mobility quadrupole/time-of-flight mass spectrometer in order to provide an …
Field-flow fractionation: New and exciting perspectives in polymer analysis
The development of advanced polymeric materials requires state-of-the-art synthesis and
molecular characterization protocols. Only the precise knowledge of molecular structure …
molecular characterization protocols. Only the precise knowledge of molecular structure …