RNA structural dynamics as captured by molecular simulations: a comprehensive overview

J Sponer, G Bussi, M Krepl, P Banáš, S Bottaro… - Chemical …, 2018 - ACS Publications
With both catalytic and genetic functions, ribonucleic acid (RNA) is perhaps the most
pluripotent chemical species in molecular biology, and its functions are intimately linked to …

How to understand atomistic molecular dynamics simulations of RNA and protein–RNA complexes?

J Šponer, M Krepl, P Banáš, P Kührová… - Wiley …, 2017 - Wiley Online Library
We provide a critical assessment of explicit‐solvent atomistic molecular dynamics (MD)
simulations of RNA and protein/RNA complexes, written primarily for non‐specialists with an …

Improving the performance of the amber RNA force field by tuning the hydrogen-bonding interactions

P Kuhrova, V Mlynsky, M Zgarbová… - Journal of chemical …, 2019 - ACS Publications
Molecular dynamics (MD) simulations became a leading tool for investigation of structural
dynamics of nucleic acids. Despite recent efforts to improve the empirical potentials (force …

Mechanistic insights into the ligand-induced unfolding of an RNA G-quadruplex

S Haldar, Y Zhang, Y **a, B Islam, S Liu… - Journal of the …, 2022 - ACS Publications
The cationic porphyrin TMPyP4 is a well-established DNA G-quadruplex (G4) binding ligand
that can stabilize different topologies via multiple binding modes. However, TMPyP4 can …

Toward convergence in folding simulations of RNA tetraloops: Comparison of enhanced sampling techniques and effects of force field modifications

V Mlynsky, M Janeček, P Kuhrova… - Journal of Chemical …, 2022 - ACS Publications
Atomistic molecular dynamics simulations represent an established technique for
investigation of RNA structural dynamics. Despite continuous development, contemporary …

Spontaneous binding of single-stranded RNAs to RRM proteins visualized by unbiased atomistic simulations with a rescaled RNA force field

M Krepl, P Pokorná, V Mlýnský… - Nucleic acids …, 2022 - academic.oup.com
Recognition of single-stranded RNA (ssRNA) by RNA recognition motif (RRM) domains is
an important class of protein–RNA interactions. Many such complexes were characterized …

Germline AGO2 mutations impair RNA interference and human neurological development

D Lessel, DM Zeitler, MRF Reijnders… - Nature …, 2020 - nature.com
ARGONAUTE-2 and associated miRNAs form the RNA-induced silencing complex (RISC),
which targets mRNAs for translational silencing and degradation as part of the RNA …

Computer folding of RNA tetraloops: identification of key force field deficiencies

P Kuhrova, RB Best, S Bottaro, G Bussi… - Journal of chemical …, 2016 - ACS Publications
The computer-aided folding of biomolecules, particularly RNAs, is one of the most difficult
challenges in computational structural biology. RNA tetraloops are fundamental RNA motifs …

Free energy landscape of GAGA and UUCG RNA tetraloops

S Bottaro, P Banáš, J Sponer… - The journal of physical …, 2016 - ACS Publications
We report the folding thermodynamics of ccUUCGgg and ccGAGAgg RNA tetraloops using
atomistic molecular dynamics simulations. We obtain a previously unreported estimation of …

UUCG RNA tetraloop as a formidable force-field challenge for MD simulations

K Mráziková, V Mlýnský, P Kührová… - Journal of chemical …, 2020 - ACS Publications
Explicit solvent atomistic molecular dynamics (MD) simulations represent an established
technique to study structural dynamics of RNA molecules and an important complement for …