Roadmap on label‐free super‐resolution imaging

VN Astratov, YB Sahel, YC Eldar… - Laser & photonics …, 2023‏ - Wiley Online Library
Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in
nanoscale objects without a need for fluorescent (FL) staining required in super‐resolved FL …

Viewing life without labels under optical microscopes

B Ghosh, K Agarwal - Communications Biology, 2023‏ - nature.com
Optical microscopes today have pushed the limits of speed, quality, and observable space in
biological specimens revolutionizing how we view life today. Further, specific labeling of …

Single-shot isotropic differential interference contrast microscopy

X Wang, H Wang, J Wang, X Liu, H Hao, YS Tan… - Nature …, 2023‏ - nature.com
Differential interference contrast (DIC) microscopy allows high-contrast, low-phototoxicity,
and label-free imaging of transparent biological objects, and has been applied in the field of …

[PDF][PDF] Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging

Y **ao, L Chen, M Pu, M Xu, Q Zhang, Y Guo… - Opto-Electronic …, 2023‏ - researching.cn
Super-resolution (SR) microscopy has dramatically enhanced our understanding of
biological processes. However, scattering media in thick specimens severely limits the …

Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments

ME Kandel, YR He, YJ Lee, THY Chen… - Nature …, 2020‏ - nature.com
Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in
cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit …

Quantitative 3D refractive index tomography of opaque samples in epi-mode

P Ledwig, FE Robles - Optica, 2020‏ - opg.optica.org
Three-dimensional (3D) refractive index (RI) tomography has recently become an exciting
new tool for biological studies. However, its limitation to (1) thin samples resulting from a …

Live-dead assay on unlabeled cells using phase imaging with computational specificity

C Hu, S He, YJ Lee, Y He, EM Kong, H Li… - Nature …, 2022‏ - nature.com
Existing approaches to evaluate cell viability involve cell staining with chemical reagents.
However, the step of exogenous staining makes these methods undesirable for rapid …

Artificial confocal microscopy for deep label-free imaging

X Chen, ME Kandel, S He, C Hu, YJ Lee, K Sullivan… - Nature …, 2023‏ - nature.com
Wide-field microscopy of optically thick specimens typically features reduced contrast due to
spatial cross-talk, in which the signal at each point in the field of view is the result of a …

Spatial light interference microscopy: principle and applications to biomedicine

X Chen, ME Kandel, G Popescu - Advances in optics and photonics, 2021‏ - opg.optica.org
In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-
shifting interferometer, built onto a phase-contrast microscope, with white-light illumination …

Cell cycle stage classification using phase imaging with computational specificity

YR He, S He, ME Kandel, YJ Lee, C Hu, N Sobh… - ACS …, 2022‏ - ACS Publications
Traditional methods for cell cycle stage classification rely heavily on fluorescence
microscopy to monitor nuclear dynamics. These methods inevitably face the typical …