Epigenetic gene regulation in plants and its potential applications in crop improvement

H Zhang, JK Zhu - Nature Reviews Molecular Cell Biology, 2024 - nature.com
DNA methylation, also known as 5-methylcytosine, is an epigenetic modification that has
crucial functions in plant growth, development and adaptation. The cellular DNA methylation …

Molecular mechanisms of the RNA polymerases in plant RNA-directed DNA methylation

G **e, X Du, H Hu, J Du - Trends in Biochemical Sciences, 2024 - cell.com
In plants, two atypical DNA-dependent RNA polymerases, RNA polymerase IV (Pol IV) and
Pol V, and an RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) together produce …

Pol IV and RDR2: a two-RNA-polymerase machine that produces double-stranded RNA

K Huang, XX Wu, CL Fang, ZG Xu, HW Zhang, J Gao… - Science, 2021 - science.org
DNA methylation affects gene expression and maintains genome integrity. The DNA-
dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RNA polymerase …

Cotranscriptional RNA processing and modification in plants

S Marquardt, E Petrillo, PA Manavella - The Plant Cell, 2023 - academic.oup.com
The activities of RNA polymerases shape the epigenetic landscape of genomes with
profound consequences for genome integrity and gene expression. A fundamental event …

[HTML][HTML] The biosynthesis process of small RNA and its pivotal roles in plant development

Q Li, Y Wang, Z Sun, H Li, H Liu - International Journal of Molecular …, 2024 - mdpi.com
In the realm of plant biology, small RNAs (sRNAs) are imperative in the orchestration of
gene expression, playing pivotal roles across a spectrum of developmental sequences and …

Structure and RNA template requirements of Arabidopsis RNA-DEPENDENT RNA POLYMERASE 2

A Fukudome, J Singh, V Mishra, E Reddem… - Proceedings of the …, 2021 - pnas.org
RNA-dependent RNA polymerases play essential roles in RNA-mediated gene silencing in
eukaryotes. In Arabidopsis, RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) physically …

RNA-directed DNA methylation mutants reduce histone methylation at the paramutated maize booster1 enhancer

I Hövel, R Bader, M Louwers, M Haring, K Peek… - Plant …, 2024 - academic.oup.com
Paramutation is the transfer of mitotically and meiotically heritable silencing information
between two alleles. With paramutation at the maize (Zea mays) booster1 (b1) locus, the low …

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

A Loffer, J Singh, A Fukudome, V Mishra, F Wang… - Elife, 2022 - elifesciences.org
In plants, selfish genetic elements, including retrotransposons and DNA viruses, are
transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short …

Dot‐blotting: a quick method for expression analysis of recombinant proteins

V Mishra - Current protocols, 2022 - Wiley Online Library
Expressing recombinant proteins in heterologous host cells is a prerequisite for purification
and other downstream processes. Cell cultures require a protein expression test to optimize …

CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing

L Felgines, B Rymen, LM Martins, G Xu… - Nature …, 2024 - nature.com
Eukaryotes must balance the need for gene transcription by RNA polymerase II (Pol II)
against the danger of mutations caused by transposable element (TE) proliferation. In plants …