Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and
express genetic information, which play central roles in diverse cellular events and diseases …

Interactions between host and bacterial cells are integral to human physiology. The
complexity of host–microbe interactions extends to different cell types, spatial aspects, and …

Simultaneous sequencing of the genome and transcriptome at the single-cell level is a
powerful tool for characterizing genomic and transcriptomic variation and revealing …

Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to
characterize cellular status, but it relies on second-strand complementary DNA (cDNA) …

Despite being a relatively recent technological development, single-cell transcriptional
analysis through high-throughput sequencing has already been used in hundreds of fruitful …

The interaction between a pathogen and a host is a highly dynamic process in which both
agents activate complex programs. Here, we introduce a single-cell RNA-sequencing …

Recent developments in microfluidic devices, nanoparticle chemistry, fluorescent
microscopy, and biochemical techniques such as genetic identification and antibody capture …

Analysis of microRNA (miRNA), a pivotal primary regulator of fundamental cellular
processes, at the single-cell level is essential to elucidate regulated gene expression …

The simultaneous quantification of multi-miRNAs in single cells reveals cellular
heterogeneity, and benefits the subtypes discrimination of cancer cells. Though micro …