Site-specific dual encoding and labeling of proteins via genetic code expansion
RM Bednar, PA Karplus, RA Mehl - Cell chemical biology, 2023 - cell.com
The ability to selectively modify proteins at two or more defined locations opens new
avenues for manipulating, engineering, and studying living systems. As a chemical biology …
avenues for manipulating, engineering, and studying living systems. As a chemical biology …
Functional analysis of protein post‐translational modifications using genetic codon expansion
Post‐translational modifications (PTMs) of proteins not only exponentially increase the
diversity of proteoforms, but also contribute to dynamically modulating the localization …
diversity of proteoforms, but also contribute to dynamically modulating the localization …
Sense codon reassignment enables viral resistance and encoded polymer synthesis
It is widely hypothesized that removing cellular transfer RNAs (tRNAs)—making their
cognate codons unreadable—might create a genetic firewall to viral infection and enable …
cognate codons unreadable—might create a genetic firewall to viral infection and enable …
Autonomous synthesis of functional, permanently phosphorylated proteins for defining the interactome of monomeric 14-3-3ζ
P Zhu, S Stanisheuski, R Franklin, A Vogel… - ACS Central …, 2023 - ACS Publications
14-3-3 proteins are dimeric hubs that bind hundreds of phosphorylated “clients” to regulate
their function. Installing stable, functional mimics of phosphorylated amino acids into …
their function. Installing stable, functional mimics of phosphorylated amino acids into …
Single-atom transition metals (Rh, Ir, Co) doped silicon carbide nanotubes (SiCNT) as nonenzymatic nitrotyrosine (NTS) sensor: Insight from theoretical calculations
GA Okon, FO Ogungbemiro, H Louis, I Benjamin… - Computational and …, 2023 - Elsevier
Abstract Transition metals Namely Rhodium (Rh), Iridium (Ir), and Cobalt (Co) doped silicon
carbide nanotube (SiCNT) surfaces were theoretically investigated using first-principles …
carbide nanotube (SiCNT) surfaces were theoretically investigated using first-principles …
Ultrafast bioorthogonal spin-labeling and distance measurements in mammalian cells using small, genetically encoded tetrazine amino acids
Site-directed spin-labeling (SDSL)─ in combination with double electron–electron
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …
Orthogonal Translation for Site-Specific Installation of Post-translational Modifications
Q Gan, C Fan - Chemical Reviews, 2024 - ACS Publications
Post-translational modifications (PTMs) endow proteins with new properties to respond to
environmental changes or growth needs. With the development of advanced proteomics …
environmental changes or growth needs. With the development of advanced proteomics …
Genetic Code Expansion History and Modern Innovations
A Costello, AA Peterson, PH Chen… - Chemical …, 2024 - ACS Publications
The genetic code is the foundation for all life. With few exceptions, the translation of nucleic
acid messages into proteins follows conserved rules, which are defined by codons that …
acid messages into proteins follows conserved rules, which are defined by codons that …
Protein tyrosine nitration in plant nitric oxide signaling
J León - Frontiers in plant science, 2022 - frontiersin.org
Nitric oxide (NO), which is ubiquitously present in living organisms, regulates many
developmental and stress-activated processes in plants. Regulatory effects exerted by NO …
developmental and stress-activated processes in plants. Regulatory effects exerted by NO …
Genetic Encoding of Phosphorylated Amino Acids into Proteins
Reversible phosphorylation is a fundamental mechanism for controlling protein function.
Despite the critical roles phosphorylated proteins play in physiology and disease, our ability …
Despite the critical roles phosphorylated proteins play in physiology and disease, our ability …