The biological function of protein, DNA, and RNA molecules often depends on relative
movements of domains with dimensions of a few nanometers. This length scale can be …

Transmembrane proteins serve important biological functions, yet precise information on
their secondary and tertiary structure is very limited. The boundaries and structures of …

Studies of structure and dynamics of proteins using site-directed spin labelling rely on
explicit modelling of spin label conformations. The large computational effort associated with …

Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-
labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the …

Using electron diffraction data corrected for diffuse scattering together with additional phase
information from 30 new images of tilted specimens, an improved experimental density map …

Ten mutants of bacteriorhodopsin, each containing a single cysteine residue regularly
spaced along helix D and facing the lipid bilayer, were derivatized with a nitroxide spin …

Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an
encyclopedic work describing the many approaches to the site-specific modification of …

Site-directed spin labeling in combination with electron paramagnetic resonance
spectroscopy has emerged as an efficient tool to elucidate the structure and conformational …

Macromolecular protein assemblies are of fundamental importance for many processes
inside the cell, as they perform complex functions and constitute central hubs where …

Substitution of a cysteine in the extracellular mouth of the pore of the Shaker-delta K+
channel permits allosteric inhibition of the channel by Zn2+ or Cd2+ ions at micromolar …