Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Binding events of ligands to receptors are the key for an understanding of biological
processes. Gaining insight into protein–protein and protein–ligand interactions in solution …

A protocol based on saturation transfer difference (STD) NMR spectra was developed to
characterize the binding interactions at an atom level, termed group epitope map** …

E3 ubiquitin ligases, which bind protein targets, leading to their ubiquitination and
subsequent degradation, are attractive drug targets due to their exquisite substrate …

Fragment-based approaches to finding novel small molecules that bind to proteins are now
firmly established in drug discovery and chemical biology. Initially developed primarily in a …

WaterLOGSY represents a powerful method for primary NMR screening in the identification
of compounds interacting with macromolecules, including proteins and DNA or RNA …

NMR spectroscopy has evolved into an important technique in support of structure-based
drug design. Here, we survey the principles that enable NMR to provide information on the …

NMR spectroscopy is currently extensively used in binding assays for hit identification, but its
use in dissociation constant determination is more limited when compared to other …

In the past decade, the potential of harnessing the ability of nuclear magnetic resonance
(NMR) spectroscopy to monitor intermolecular interactions as a tool for drug discovery has …

Ligand-based fluorine NMR screening has gained popularity in drug discovery projects
during the past decade and has become a powerful methodology to produce high quality …