Prime editing for precise and highly versatile genome manipulation
Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
High-efficiency prime editing with optimized, paired pegRNAs in plants
Prime editing (PE) applications are limited by low editing efficiency. Here we show that
designing prime binding sites with a melting temperature of 30° C leads to optimal …
designing prime binding sites with a melting temperature of 30° C leads to optimal …
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement
Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
[HTML][HTML] CRISPR/Cas: A powerful tool for gene function study and crop improvement
Background It is a long-standing goal of scientists and breeders to precisely control a gene
for studying its function as well as improving crop yield, quality, and tolerance to various …
for studying its function as well as improving crop yield, quality, and tolerance to various …
Increasing the efficiency and precision of prime editing with guide RNA pairs
The recently reported prime editor (PE) can produce all types of base substitution, insertion
and deletion, greatly expanding the scope of genome editing. However, improving the …
and deletion, greatly expanding the scope of genome editing. However, improving the …
Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)
Prime editors (PEs), which can install desired base edits without donor DNA or double-
strand breaks, have been used in plants and can, in principle, accelerate crop improvement …
strand breaks, have been used in plants and can, in principle, accelerate crop improvement …
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
YY Jiang, YP Chai, MH Lu, XL Han, Q Lin, Y Zhang… - Genome biology, 2020 - Springer
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing
technology that has been recently developed. However, low efficiency of prime editing has …
technology that has been recently developed. However, low efficiency of prime editing has …
[HTML][HTML] TALE and TALEN genome editing technologies
S Becker, J Boch - Gene and Genome Editing, 2021 - Elsevier
TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …
editing revolution. Their application in multiple species brought targeted mutagenesis to the …