Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks

R Nyaruaba, C Mwaliko, D Dobnik… - Clinical Microbiology …, 2022 - journals.asm.org
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health …

[HTML][HTML] RT-qPCR testing and performance metrics in the COVID-19 era

SA Bustin - International Journal of Molecular Sciences, 2024 - mdpi.com
The COVID-19 pandemic highlighted the crucial role of diagnostic testing in managing
infectious diseases, particularly through the use of reverse transcription-quantitative …

Diagnostic, prognostic, and therapeutic value of droplet digital PCR (ddPCR) in COVID-19 patients: A systematic review

A Ishak, MM AlRawashdeh, SM Esagian… - Journal of Clinical …, 2021 - mdpi.com
Accurate detection of SARS-CoV-2, the pathogen causing the global pandemic of COVID-
19, is essential for disease surveillance and control. Quantitative reverse transcription PCR …

Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution

B Li, B Lin, Y Wang, Y Shi, W Zeng, Y Zhao… - Nature …, 2024 - nature.com
Highly sensitive airborne virus monitoring is critical for preventing and containing epidemics.
However, the detection of airborne viruses at ultra-low concentrations remains challenging …

Performance verification of five commercial RT-qPCR diagnostic kits for SARS-CoV-2

M Yang, S Cao, Y Liu, Z Zhang, R Zheng, Y Li… - Clinica Chimica …, 2022 - Elsevier
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has
caused a global pandemic beginning in 2020, can be detected by reverse-transcription …

Recent progresses in electrochemical DNA biosensors for SARS-CoV-2 detection

Y Mei, X Lin, C He, W Zeng, Y Luo, C Liu… - … in Bioengineering and …, 2022 - frontiersin.org
Coronavirus disease 19 (COVID-19) is still a major public health concern in many nations
today. COVID-19 transmission is now controlled mostly through early discovery, isolation …

CRISPR/Cas12a-based versatile method for checking quantitative polymerase chain reaction samples with cycles of threshold values in the gray zone

Y Chen, S Wu, H Wu, P Cheng, X Wang, S Qian… - ACS …, 2021 - ACS Publications
Quantitative polymerase chain reaction (qPCR) is widely applied in foodborne pathogen
detection and diagnosis. According to the cycles of threshold (Ct) values of qPCR testing …

Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR

C Niu, X Wang, Y Zhang, L Lu, D Wang, Y Gao… - Analytical and …, 2021 - Springer
The pandemic of the novel coronavirus disease 2019 (COVID-19) has caused severe harm
to the health of people all around the world. Molecular detection of the pathogen, severe …

A two-step process for in silico screening to assess the performance of qRTPCR kits against variant strains of SARS-CoV-2

S Gupta, A Kumar, N Gupta, DR Bharti, N Aggarwal… - BMC genomics, 2022 - Springer
Background Since inception of the COVID-19 pandemic, early detection and isolation of
positive cases is one of the key strategies to restrict disease transmission. Real time reverse …

The role of subgenomic RNA in discordant results from reverse transcription-polymerase chain reaction tests for COVID-19

NB Top**s, LK Oberding, YC Lin… - … of Pathology & …, 2022 - meridian.allenpress.com
Context.—Reverse transcription–polymerase chain reaction (RT-PCR) is the standard
method of diagnosing COVID-19. An inconclusive test result occurs when 1 RT-PCR target …