A conceptual framework for understanding phase separation and addressing open questions and challenges
Macromolecular phase separation is being recognized for its potential importance and
relevance as a driver of spatial organization within cells. Here, we describe a framework …
relevance as a driver of spatial organization within cells. Here, we describe a framework …
Phase transitions of associative biomacromolecules
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …
biomacromolecules, provide the driving forces for the formation and compositional …
Modulating biomolecular condensates: a novel approach to drug discovery
In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …
been reported to play key roles in many cellular functions by compartmentalizing specific …
Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions
Macromolecular phase separation is thought to be one of the processes that drives the
formation of membraneless biomolecular condensates in cells. The dynamics of phase …
formation of membraneless biomolecular condensates in cells. The dynamics of phase …
A brief guideline for studies of phase-separated biomolecular condensates
Y Gao, X Li, P Li, Y Lin - Nature Chemical Biology, 2022 - nature.com
Cells are exquisitely compartmentalized to achieve precise spatiotemporal regulation of
myriad processes and pathways. Phase separation offers one way to achieve territorial …
myriad processes and pathways. Phase separation offers one way to achieve territorial …
Droplet physics and intracellular phase separation
Living cells are spatially organized by compartments that can nucleate, grow, and dissolve.
Compartmentalization can emerge by phase separation, leading to the formation of droplets …
Compartmentalization can emerge by phase separation, leading to the formation of droplets …
A solid beta-sheet structure is formed at the surface of FUS droplets during aging
Phase transitions are important to understand cell dynamics, and the maturation of liquid
droplets is relevant to neurodegenerative disorders. We combined NMR and Raman …
droplets is relevant to neurodegenerative disorders. We combined NMR and Raman …
Mesoscale structure–function relationships in mitochondrial transcriptional condensates
In live cells, phase separation is thought to organize macromolecules into membraneless
structures known as biomolecular condensates. Here, we reconstituted transcription in …
structures known as biomolecular condensates. Here, we reconstituted transcription in …
Targeting nucleic acid phase transitions as a mechanism of action for antimicrobial peptides
Antimicrobial peptides (AMPs), which combat bacterial infections by disrupting the bacterial
cell membrane or interacting with intracellular targets, are naturally produced by a number of …
cell membrane or interacting with intracellular targets, are naturally produced by a number of …
ISO-FLUCS: symmetrization of optofluidic manipulations in quasi-isothermal micro-environments
Recently, it has been demonstrated that thermoviscous flows can be used for a range of fine
micromanipulations, such as moving the cytoplasm of cells and develo** embryos …
micromanipulations, such as moving the cytoplasm of cells and develo** embryos …