CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

C Li, E Brant, H Budak, B Zhang - Journal of Zhejiang University-SCIENCE …, 2021 - Springer
Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

SY Wang, YC Du, DX Wang, JY Ma, AN Tang… - Analytica Chimica …, 2021 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …

A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics

F Hu, Y Liu, S Zhao, Z Zhang, X Li, N Peng… - Biosensors and …, 2022 - Elsevier
The pandemic due to the outbreak of 2019 coronavirus disease (COVID-19) caused by
novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has raised significant …

Efficient, rapid, and sensitive detection of plant RNA viruses with one-pot RT-RPA–CRISPR/Cas12a assay

R Aman, A Mahas, T Marsic, N Hassan… - Frontiers in …, 2020 - frontiersin.org
Most viruses that infect plants use RNA to carry their genomic information; timely and robust
detection methods are crucial for efficient control of these diverse pathogens. The RNA …

Emerging technologies revolutionising disease diagnosis and monitoring in aquatic animal health

K Bohara, P Joshi, KP Acharya… - Reviews in …, 2024 - Wiley Online Library
In recent years, aquaculture has seen tremendous growth worldwide due to technological
advancements, leading to research and development of various innovations. Aquaculture …

Pyrococcus furiosus Argonaute Combined with Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Enterocytozoon hepatopenaei

L Yang, B Guo, Y Wang, C Zhao, X Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Enterocytozoon hepatopenaei (EHP) is one of the most serious pathogens in shrimp
farming. This study combines recombinase polymerase amplification (RPA) with the …

Development of CRISPR/Cas13a-based assays for the diagnosis of schistosomiasis

SR MacGregor, DP McManus, H Sivakumaran… - …, 2023 - thelancet.com
Background Schistosomiasis is a disease that significantly impacts human health in the
develo** world. Effective diagnostics are urgently needed for improved control of this …

Applications of the versatile CRISPR‐Cas13 RNA targeting system

M Kordyś, R Sen, Z Warkocki - Wiley Interdisciplinary Reviews …, 2022 - Wiley Online Library
CRISPR‐Cas are adaptable natural prokaryotic defense systems that act against invading
viruses and plasmids. Among the six currently known major CRISPR‐Cas types, the type VI …

The shrimp microsporidian Enterocytozoon hepatopenaei (EHP): Biology, pathology, diagnostics and control

T Chaijarasphong, N Munkongwongsiri… - Journal of invertebrate …, 2021 - Elsevier
Disease is a major limiting factor in the global production of cultivated shrimp. The
microsporidian parasite Enterocytozoon hepatopenaei (EHP) was formally characterized in …

From the basics to emerging diagnostic technologies: What is on the horizon for tilapia disease diagnostics?

HT Dong, T Chaijarasphong, AC Barnes… - Reviews in …, 2023 - Wiley Online Library
Tilapia is an affordable protein source farmed in over 140 countries with the majority of
production in low‐and middle‐income countries. Intensification of tilapia farming has …