Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
[HTML][HTML] CRISPR/Cas genome editing in soybean: Challenges and new insights to overcome existing bottlenecks
NS Freitas-Alves, CE Moreira-Pinto… - Journal of Advanced …, 2024 - Elsevier
Background Soybean is a worldwide-cultivated crop due to its applications in the food, feed,
and biodiesel industries. Genome editing in soybean began with ZFN and TALEN …
and biodiesel industries. Genome editing in soybean began with ZFN and TALEN …
Lachnospiraceae are emerging industrial biocatalysts and biotherapeutics
The Lachnospiraceae is a family of anaerobic bacteria in the class Clostridia with potential
to advance the bio-economy and intestinal therapeutics. Some species of Lachnospiraceae …
to advance the bio-economy and intestinal therapeutics. Some species of Lachnospiraceae …
Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …
sourced from a human pathogen, is the most widely used. Here, through in silico data …
Optimizing ErCas12a for efficient gene editing in Arabidopsis thaliana
J Pietralla, N Capdeville, P Schindele… - Plant Biotechnology …, 2024 - Wiley Online Library
Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …
Engineering a robust Cas12i3 variant‐mediated wheat genome editing system
W Wang, L Yan, J Li, C Zhang, Y He… - Plant Biotechnology …, 2024 - Wiley Online Library
Summary Wheat (Triticum aestivum L., 2 n= 6 x= 42, AABBDD) is one of the most important
food crops in the world. CRISPR/Cas12i3, which belongs to the type V‐I Cas system, has …
food crops in the world. CRISPR/Cas12i3, which belongs to the type V‐I Cas system, has …
Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants
Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been
widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique …
widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique …
The type V effectors for CRISPR/Cas-mediated genome engineering in plants
R Zhang, N Chai, T Liu, Z Zheng, Q Lin, X **e… - Biotechnology …, 2024 - Elsevier
A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed
as gene editing tools. Among these, Cas12 effectors developed based on Class II type V …
as gene editing tools. Among these, Cas12 effectors developed based on Class II type V …
Cas12a and MAD7, genome editing tools for breeding
S Hozumi, YC Chen, T Takemoto… - Breeding …, 2024 - jstage.jst.go.jp
Food shortages due to population growth and climate change are expected to occur in the
near future as a problem that urgently requires solutions. Conventional breeding techniques …
near future as a problem that urgently requires solutions. Conventional breeding techniques …
PAM‐relaxed and temperature‐tolerant CRISPR‐Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and …
S Liu, Y He, T Fan, M Zhu, C Qi, Y Ma… - Plant Biotechnology …, 2025 - Wiley Online Library
Summary Class 2 Type V‐A CRISPR‐Cas (Cas12a) nucleases are powerful genome editing
tools, particularly effective in A/T‐rich genomic regions, complementing the widely used …
tools, particularly effective in A/T‐rich genomic regions, complementing the widely used …