Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

A molecular motor is made of either a single macromolecule or a macromolecular complex.
Just like their macroscopic counterparts, molecular motors “transduce” input energy into …

Probing the oligomeric state of abundant molecules, such as membrane proteins in intact
cells, is essential, but has not been straightforward. We address this challenge with a simple …

The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of
fluorescence-based assays to study biological entities and interactions. Especially the …

In recent years, single-molecule and single-particle fluorescence microscopy has emerged
as a tool to investigate chemical systems. After an initial lag of over a decade with respect to …

Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular
distances and interactions at the nanoscale level. The strong dependence of transfer …

Cancer is a complex and heterogeneous disease that exhibits high levels of robustness
against various therapeutic interventions. It is a constellation of diverse and evolving …

In the past three decades, the ability to optically manipulate biomolecules has spurred a new
era of medical and biophysical research. Optical tweezers (OT) have enabled experimenters …

The surfaces of colloidal nanocrystals are complex interfaces between solid crystals,
coordinating ligands, and liquid solutions. For fluorescent quantum dots, the properties of …

Advanced microscopy methods allow obtaining information on (dynamic) conformational
changes in biomolecules via measuring a single molecular distance in the structure. It is …