Nucleic acid joining enzymes: biological functions and synthetic applications beyond DNA

C Blackstock, C Walters-Freke, N Richards… - Biochemical …, 2025‏ - portlandpress.com
DNA-joining by ligase and polymerase enzymes has provided the foundational tools for
generating recombinant DNA and enabled the assembly of gene and genome-sized …

Research Progress on the Assembly of Large DNA Fragments

W Tan, Q Miao, X Jia, Y Liu, S Li, D Yang - ChemBioChem, 2024‏ - Wiley Online Library
Synthetic biology, a newly and rapidly develo** interdisciplinary field, has demonstrated
increasing potential for extensive applications in the wide areas of biomedicine, biofuels …

Benzoyl and pivaloyl as efficient protecting groups for controlled enzymatic synthesis of DNA and XNA oligonucleotides

M Flamme, D Katkevica, K Pajuste… - Asian Journal of …, 2022‏ - Wiley Online Library
Controlled enzymatic synthesis is an alluring alternative to solid‐phase synthesis and
polymerase‐mediated incorporation of nucleotides for the crafting of chemically modified …

Enzymatic synthesis of vancomycin-modified DNA

C Figazzolo, F Bonhomme, S Saidjalolov… - Molecules, 2022‏ - mdpi.com
Many potent antibiotics fail to treat bacterial infections due to emergence of drug-resistant
strains. This surge of antimicrobial resistance (AMR) calls in for the development of …

Ferrocene as a potential electrochemical reporting surrogate of abasic sites in DNA

C Figazzolo, Y Ma, JHR Tucker… - Organic & Biomolecular …, 2022‏ - pubs.rsc.org
Methods for the real-time monitoring of the substrate acceptance of modified nucleotides by
DNA polymerases are in high demand. In a step towards this aim, we have incorporated …

Ferrocene as an electrochemical reporting surrogate of abasic sites in DNA

C Figazzolo, Y Ma, J Tucker, M Hollenstein - 2022‏ - chemrxiv.org
Methods for the real-time monitoring of the substrate acceptance of modified nucleotides by
DNA polymerases are in high demand. In a step towards this aim, we have incorporated …