Gene expression involves transcription, translation and the turnover of mRNAs and proteins.
The degree to which protein abundances scale with mRNA levels and the implications in …

The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Chromosome structure in mammals is thought to regulate transcription by modulating three-
dimensional interactions between enhancers and promoters, notably through CTCF …

Epithelial–mesenchymal plasticity (EMP) enables cells to interconvert between several
states across the epithelial–mesenchymal landscape, thereby acquiring hybrid epithelial …

Exosomes are small, single-membrane, secreted organelles of∼ 30 to∼ 200 nm in
diameter that have the same topology as the cell and are enriched in selected proteins …

RNA velocity has enabled the recovery of directed dynamic information from single‐cell
transcriptomics by connecting measurements to the underlying kinetics of gene expression …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

Intestinal organoids are complex three-dimensional structures that mimic the cell-type
composition and tissue organization of the intestine by recapitulating the self-organizing …

Therapies that target signalling molecules that are mutated in cancers can often have
substantial short-term effects, but the emergence of resistant cancer cells is a major barrier …

We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of
RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales …