Stable isotope-labeling studies in metabolomics: new insights into structure and dynamics of metabolic networks

A Chokkathukalam, DH Kim, MP Barrett, R Breitling… - Bioanalysis, 2014 - Taylor & Francis
The rapid emergence of metabolomics has enabled system-wide measurements of
metabolites in various organisms. However, advances in the mechanistic understanding of …

How to measure metabolic fluxes: a taxonomic guide for 13C fluxomics

S Niedenführ, W Wiechert, K Nöh - Current opinion in biotechnology, 2015 - Elsevier
Highlights•Tracer experiments, analytics and modeling constitute the 13 C fluxomics
triumvirate.•13 C fluxomics tools differ in complexity, information power and universality.•The …

The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene

W **ong, JA Morgan, J Ungerer, B Wang, PC Maness… - Nature Plants, 2015 - nature.com
The cyanobacterial tricarboxylic acid (TCA) cycle functions in both in biosynthesis and
energy generation. However, it has until recently been generally considered to be …

Isotopically nonstationary metabolic flux analysis (INST-MFA): putting theory into practice

YE Cheah, JD Young - Current Opinion in Biotechnology, 2018 - Elsevier
Typically, 13 C flux analysis relies on assumptions of both metabolic and isotopic steady
state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate …

Isotope tracing untargeted metabolomics reveals macrophage polarization-state-specific metabolic coordination across intracellular compartments

P Puchalska, X Huang, SE Martin, X Han, GJ Patti… - Iscience, 2018 - cell.com
We apply stable isotope tracing, mass-spectrometry-based untargeted metabolomics, to
reveal the biochemical space labeled by 13 C-substrates in bone-marrow-derived …

An improved method for extraction of polar and charged metabolites from cyanobacteria

CB Prasannan, D Jaiswal, R Davis, PP Wangikar - PLoS One, 2018 - journals.plos.org
A key requirement for 13C Metabolic flux analysis (13C-MFA), a widely used technique to
estimate intracellular metabolic fluxes, is an efficient method for the extraction of …

Reverse engineering the cancer metabolic network using flux analysis to understand drivers of human disease

MG Badur, CM Metallo - Metabolic engineering, 2018 - Elsevier
Metabolic dysfunction has reemerged as an essential hallmark of tumorigenesis, and
metabolic phenotypes are increasingly being integrated into pre-clinical models of disease …

Advances in metabolic flux analysis toward genome-scale profiling of higher organisms

G Basler, AR Fernie, Z Nikoloski - Bioscience Reports, 2018 - portlandpress.com
Methodological and technological advances have recently paved the way for metabolic flux
profiling in higher organisms, like plants. However, in comparison with omics technologies …

Isotope-assisted metabolic flux analysis: a powerful technique to gain new insights into the human metabolome in health and disease

B Moiz, A Li, S Padmanabhan, G Sriram, AM Clyne - Metabolites, 2022 - mdpi.com
Cell metabolism represents the coordinated changes in genes, proteins, and metabolites
that occur in health and disease. The metabolic fluxome, which includes both intracellular …

Metabolic Flux Analysis of the Synechocystis sp. PCC 6803 ΔnrtABCD Mutant Reveals a Mechanism for Metabolic Adaptation to Nitrogen-Limited Conditions

T Nakajima, K Yoshikawa, Y Toya… - Plant and Cell …, 2017 - academic.oup.com
Metabolic flux redirection during nitrogen-limited growth was investigated in the
Synechocystis sp. PCC 6803 glucose-tolerant (GT) strain under photoautotrophic conditions …