Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy

E Orsi, PI Nikel, LK Nielsen, S Donati - Nature Communications, 2023 - nature.com
A true circular carbon economy must upgrade waste greenhouse gases. C1-based
biomanufacturing is an attractive solution, in which one carbon (C1) molecules (eg CO2 …

Metabolomics and microbial metabolism: toward a systematic understanding

D Holbrook-Smith, J Trouillon… - Annual Review of …, 2023 - annualreviews.org
Over the past decades, our understanding of microbial metabolism has increased
dramatically. Metabolomics, a family of techniques that are used to measure the quantities of …

Metabolomics-based development of bioproduction processes toward industrial-scale production

K Tanaka, T Bamba, A Kondo, T Hasunuma - Current Opinion in …, 2024 - Elsevier
Highlights•Metabolomics accelerates bioproduction via strain, culture, and model
improvement.•Metabolomics is a powerful driving force for the DBTL-based strain …

Understanding the stringent response: experimental context matters

J Dworkin - Mbio, 2023 - journals.asm.org
As rapidly growing bacteria begin to exhaust essential nutrients, they enter a state of
reduced growth, ultimately leading to stasis or quiescence. Investigation of the response to …

Optimized Fast Filtration-Based Sampling and Extraction Enables Precise and Absolute Quantification of the Escherichia coli Central Carbon Metabolome

LB Thorfinnsdottir, L García-Calvo, GH Bø, P Bruheim… - Metabolites, 2023 - mdpi.com
Precise and accurate quantification is a prerequisite for interpretation of targeted
metabolomics data, but this task is challenged by the inherent instability of the analytes. The …

Coenzyme biosynthesis in response to precursor availability reveals incorporation of β-alanine from pantothenate in prototrophic bacteria

B Ryback, JA Vorholt - Journal of Biological Chemistry, 2023 - jbc.org
Coenzymes are important for all classes of enzymatic reactions and essential for cellular
metabolism. Most coenzymes are synthesized from dedicated precursors, also referred to as …

Optogenetic manipulation of cellular energetics in Escherichia coli

T Schmidlechner - 2024 - era.ed.ac.uk
Optogenetics is a powerful research tool due to the unrivalled spatiotemporal precision and
non-invasiveness of light. Proteorhodopsin from SAR86 γ-proteobacterium (PR), a light …