Exploring free-energy landscapes of intrinsically disordered proteins at atomic resolution using NMR spectroscopy

MR Jensen, M Zweckstetter, J Huang… - Chemical …, 2014 - ACS Publications
The last 15 years have seen a paradigm shift in our understanding of protein biochemistry,
with the realization that an unexpectedly high fraction of the human genome codes for …

Describing intrinsically disordered proteins at atomic resolution by NMR

MR Jensen, RWH Ruigrok, M Blackledge - Current opinion in structural …, 2013 - Elsevier
There is growing interest in the development of physical methods to study the
conformational behaviour and biological activity of intrinsically disordered proteins (IDPs). In …

Disease-specific tau filaments assemble via polymorphic intermediates

S Lövestam, D Li, JL Wagstaff, A Kotecha, D Kimanius… - Nature, 2024 - nature.com
Intermediate species in the assembly of amyloid filaments are believed to play a central role
in neurodegenerative diseases and may constitute important targets for therapeutic …

A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain

P Mohanty, J Shenoy, A Rizuan… - Proceedings of the …, 2023 - National Acad Sciences
TAR DNA-binding protein 43 (TDP-43) is involved in key processes in RNA metabolism and
is frequently implicated in many neurodegenerative diseases, including amyotrophic lateral …

Extreme disorder in an ultrahigh-affinity protein complex

A Borgia, MB Borgia, K Bugge, VM Kissling… - Nature, 2018 - nature.com
Molecular communication in biology is mediated by protein interactions. According to the
current paradigm, the specificity and affinity required for these interactions are encoded in …

ALS mutations disrupt phase separation mediated by α-helical structure in the TDP-43 low-complexity C-terminal domain

AE Conicella, GH Zerze, J Mittal, NL Fawzi - Structure, 2016 - cell.com
RNA-binding protein TDP-43 mediates essential RNA processing but forms cytoplasmic
neuronal inclusions via its C-terminal domain (CTD) in amyotrophic lateral sclerosis (ALS). It …

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

AE Conicella, GL Dignon, GH Zerze… - Proceedings of the …, 2020 - National Acad Sciences
Liquid–liquid phase separation (LLPS) is involved in the formation of membraneless
organelles (MLOs) associated with RNA processing. The RNA-binding protein TDP-43 is …

TAR DNA-binding protein 43 (TDP-43) liquid–liquid phase separation is mediated by just a few aromatic residues

HR Li, WC Chiang, PC Chou, WJ Wang… - Journal of Biological …, 2018 - ASBMB
Eukaryotic cells contain distinct organelles, but not all of these compartments are enclosed
by membranes. Some intrinsically disordered proteins mediate membraneless organelle …

Flexible-meccano: a tool for the generation of explicit ensemble descriptions of intrinsically disordered proteins and their associated experimental observables

V Ozenne, F Bauer, L Salmon, J Huang… - …, 2012 - academic.oup.com
Abstract Motivation: Intrinsically disordered proteins (IDPs) represent a significant fraction of
the human proteome. The classical structure function paradigm that has successfully …

Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation

RD Elias, Y Zhu, Q Su, R Ghirlando, J Zhang… - Science …, 2023 - science.org
Cytokinetic abscission, the last step of cell division, is regulated by the ESCRT machinery. In
response to mitotic errors, ESCRT proteins, namely, ALIX, CHMP4B, and CHMP4C …