Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

The cell biologist's guide to super-resolution microscopy

G Jacquemet, AF Carisey, H Hamidi… - Journal of Cell …, 2020 - journals.biologists.com
Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Magnify is a universal molecular anchoring strategy for expansion microscopy

A Klimas, BR Gallagher, P Wijesekara, S Fekir… - Nature …, 2023 - nature.com
Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

HGJ Damstra, B Mohar, M Eddison, A Akhmanova… - Elife, 2022 - elifesciences.org
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …

Click-ExM enables expansion microscopy for all biomolecules

D Sun, X Fan, Y Shi, H Zhang, Z Huang, B Cheng… - Nature …, 2021 - nature.com
Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …

Light microscopy of proteins in their ultrastructural context

O M'Saad, J Bewersdorf - Nature communications, 2020 - nature.com
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Lipid expansion microscopy

BM White, P Kumar, AN Conwell, K Wu… - Journal of the …, 2022 - ACS Publications
Strategies to visualize cellular membranes with light microscopy are restricted by the
diffraction limit of light, which far exceeds the dimensions of lipid bilayers. Here, we describe …

Expansion microscopy of C. elegans

CC Yu, NC Barry, AT Wassie, A Sinha, A Bhattacharya… - Elife, 2020 - elifesciences.org
We recently developed expansion microscopy (ExM), which achieves nanoscale-precise
imaging of specimens at~ 70 nm resolution (with~ 4.5 x linear expansion) by isotropic …

Synergistic pharmacological therapy to modulate glial cells in spinal cord injury

V Veneruso, E Petillo, F Pizzetti, A Orro… - Advanced …, 2024 - Wiley Online Library
Current treatments for modulating the glial‐mediated inflammatory response after spinal
cord injury (SCI) have limited ability to improve recovery. This is quite likely due to the lack of …