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Current progress in expansion microscopy: chemical strategies and applications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …
visualization of biological targets at nanoscale resolution on conventional fluorescence …
The cell biologist's guide to super-resolution microscopy
Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …
specific molecular components within cells. However, the resolution of light microscopy is …
Magnify is a universal molecular anchoring strategy for expansion microscopy
Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …
and isotropically magnifying preserved biological specimens embedded in a crosslinked …
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …
Click-ExM enables expansion microscopy for all biomolecules
Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …
Light microscopy of proteins in their ultrastructural context
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …
Expansion microscopy of C. elegans
We recently developed expansion microscopy (ExM), which achieves nanoscale-precise
imaging of specimens at~ 70 nm resolution (with~ 4.5 x linear expansion) by isotropic …
imaging of specimens at~ 70 nm resolution (with~ 4.5 x linear expansion) by isotropic …
Expanding boundaries–a cell biologist's guide to expansion microscopy
N Hümpfer, R Thielhorn, H Ewers - Journal of Cell Science, 2024 - journals.biologists.com
Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in
light microscopy. In contrast to super-resolution microscopy methods that rely on …
light microscopy. In contrast to super-resolution microscopy methods that rely on …
Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding
Proteins are densely packed in cells and tissues, where they form complex nanostructures.
Expansion microscopy (ExM) variants have been used to separate proteins from each other …
Expansion microscopy (ExM) variants have been used to separate proteins from each other …
Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid
Malaria is caused by unicellular Plasmodium parasites. Plasmodium relies on diverse
microtubule cytoskeletal structures for its reproduction, multiplication, and dissemination …
microtubule cytoskeletal structures for its reproduction, multiplication, and dissemination …