Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

The cell biologist's guide to super-resolution microscopy

G Jacquemet, AF Carisey, H Hamidi… - Journal of Cell …, 2020 - journals.biologists.com
Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Magnify is a universal molecular anchoring strategy for expansion microscopy

A Klimas, BR Gallagher, P Wijesekara, S Fekir… - Nature …, 2023 - nature.com
Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

HGJ Damstra, B Mohar, M Eddison, A Akhmanova… - Elife, 2022 - elifesciences.org
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …

Click-ExM enables expansion microscopy for all biomolecules

D Sun, X Fan, Y Shi, H Zhang, Z Huang, B Cheng… - Nature …, 2021 - nature.com
Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence
microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM …

Light microscopy of proteins in their ultrastructural context

O M'Saad, J Bewersdorf - Nature communications, 2020 - nature.com
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Expansion microscopy of C. elegans

CC Yu, NC Barry, AT Wassie, A Sinha, A Bhattacharya… - Elife, 2020 - elifesciences.org
We recently developed expansion microscopy (ExM), which achieves nanoscale-precise
imaging of specimens at~ 70 nm resolution (with~ 4.5 x linear expansion) by isotropic …

Expanding boundaries–a cell biologist's guide to expansion microscopy

N Hümpfer, R Thielhorn, H Ewers - Journal of Cell Science, 2024 - journals.biologists.com
Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in
light microscopy. In contrast to super-resolution microscopy methods that rely on …

Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding

PA Valdes, CC Yu, J Aronson, D Ghosh… - Science translational …, 2024 - science.org
Proteins are densely packed in cells and tissues, where they form complex nanostructures.
Expansion microscopy (ExM) variants have been used to separate proteins from each other …

Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid

E Bertiaux, AC Balestra, L Bournonville, V Louvel… - PLoS …, 2021 - journals.plos.org
Malaria is caused by unicellular Plasmodium parasites. Plasmodium relies on diverse
microtubule cytoskeletal structures for its reproduction, multiplication, and dissemination …