Nonenzymatic glycation of proteins, peptides and other macromolecules (the Maillard
reaction) has been implicated in a number of pathologies, most clearly in diabetes mellitus …

Insulin is a small protein crucial for regulating the blood glucose level in all animals. Since
1922 it has been used for the treatment of patients with diabetes. Despite consisting of just …

Labeling of native proteins invites interest from diverse segments of science. However, there
remains the significant unmet challenge in precise labeling at a single site of a protein. Here …

Proteins, the major functional macromolecules of life, are composed of long chains of L-α-
amino acids linked by amide bonds between the α-caboxyl group of one residue and the α …

Paramount efforts by pharmaceutical industry to identify new targets for obesity-diabetes
(Diabesity) pharmacological intervention have led to a number of agents developed and …

Bovine insulin was glycated under hyperglycemic reducing conditions and in nonreducing
conditions. Purification through HPLC allowed isolating glycated forms of insulin and a novel …

Aims/hypothesis Glycation of insulin, resulting in impaired bioactivity, has been shown within
pancreatic beta cells. We have used a novel and specific radioimmunoassay to detect …

Non-enzymatic glycation of protein is mediated via an interaction between the aldehyde
group of a reducing sugar and available α-or ε-amino moieties of the protein. The above …

The reaction of peptide Gly-Ala-Phe with the α-dicarbonyl compounds glyoxal and
methylglyoxal was studied under physiological conditions (pH= 7.4, 37° C). Using HPLC …

Parenteral-nutrition (PN)-induced hyperglycemia increases morbidity and mortality and must
be treated with insulin. Unfortunately, the addition of insulin to a ternary PN admixture leads …