Multicellular organisms are composed of diverse cell types that must coordinate their
behaviors through communication. Cell–cell communication (CCC) is essential for growth …

Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

Protein interaction networks and protein compartmentalization underlie all signaling and
regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a …

Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the
proteomic analysis of subcellular regions difficult or impossible to access by conventional …

Cells operate through protein interaction networks organized in space and time. Here, we
describe an approach to resolve both dimensions simultaneously by using proximity …

DNA–protein interactions regulate critical biological processes. Identifying proteins that bind
to specific, functional genomic loci is essential to understand the underlying regulatory …

Lipid droplet (LD) functions are regulated by a complement of integral and peripheral
proteins that associate with the bounding LD phospholipid monolayer. Defining the …

BioID is a unique method to screen for physiologically relevant protein interactions that
occur in living cells. This technique harnesses a promiscuous biotin ligase to biotinylate …

BioID is a well-established method for identifying protein–protein interactions and has been
utilized within live cells and several animal models. However, the conventional labeling …

The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …