The separation sciences, the front end to proteomics: An historical perspective

EC Nice - Biomedical Chromatography, 2021 - Wiley Online Library
It is now over 25 years since the term proteomics (analysis of the entire protein complement
of a cell, tissue, or organism under a specific, defined set of conditions) was originally …

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

U Andjelković, S Tufegdžić, M Popović - Electrophoresis, 2017 - Wiley Online Library
The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low
back pressure, easy preparation process and miniaturisation, and the availability of different …

Experimental study and modeling of the influence of mixed electrolytes on adsorption of macromolecules on a hydrophobic resin

A Werner, H Hasse - Journal of chromatography A, 2013 - Elsevier
The influence of mixed electrolytes on the adsorption of macromolecules on a hydrophobic
resin is explored. The macromolecules are native lysozyme, di-PEG-lysozyme-5kDa and …

Low-cost purification of nisin from milk whey to a highly active product

AF Jozala, LC de Lencastre Novaes… - Food and bioproducts …, 2015 - Elsevier
Nisin is a natural peptide used as a preservative in a variety of food products, in which it
inhibits mainly Gram-positive bacterial growth, including multidrug-resistant pathogens …

Influence of mixed electrolytes on the adsorption of lysozyme, PEG, and PEGylated lysozyme on a hydrophobic interaction chromatography resin

E Hackemann, A Werner, H Hasse - Biotechnology Progress, 2017 - Wiley Online Library
In a recent work (Werner A and Hasse H in J Chromatogr A 2013; 1315: 135) the influence
of mixed electrolytes on the adsorption of the macromolecules lysozyme, PEG and di …

Prediction of the elution profiles of proteins in mixed salt systems in hydrophobic interaction chromatography

N Galeotti, E Hackemann, F Jirasek, H Hasse - Separation and Purification …, 2020 - Elsevier
Hydrophobic interaction chromatography (HIC) is often used for purifying proteins. A
mathematical model to describe the complex effects of salts on the adsorption equilibria in …

Sample displacement chromatography of plasmid DNA isoforms

U Černigoj, U Martinuč, S Cardoso, NL Krajnc… - … of Chromatography A, 2015 - Elsevier
Sample displacement chromatography (SDC) is a chromatographic technique that utilises
different relative binding affinities of components in a sample mixture and has been widely …

Salt‐tolerant cation exchanger‐containing sulfate groups as a viable alternative for mixed‐mode type and heparin‐based affinity resins

M Begić, S Pečenković, MŠ Gajdošik… - Biotechnology …, 2021 - Wiley Online Library
Ion‐exchange chromatography is still one of the most popular protein separation
techniques. Before chromatographic separation, the high salt concentration in various …

Polymethacrylate‐based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes

T Martinović, D Josić - Electrophoresis, 2017 - Wiley Online Library
The experiences in the production and application of polymethacrylate‐based monolithic
supports, since their development almost thirty years ago, are presented. The main driving …

An alternative method for serum protein depletion/enrichment by precipitation at mildly acidic pH values and low ionic strength

AK Henning, D Albrecht, K Riedel, TC Mettenleiter… - …, 2015 - Wiley Online Library
Serum proteome analysis is severely hampered by the extreme dynamic range of protein
concentrations, but tools for the specific depletion of highly abundant serum proteins lack for …