A user's guide to golden gate cloning methods and standards

JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Making small molecules in plants: a chassis for synthetic biology‐based production of plant natural products

X Liu, P Zhang, Q Zhao… - Journal of Integrative Plant …, 2023 - Wiley Online Library
Plant natural products have been extensively exploited in food, medicine, flavor, cosmetic,
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …

CRISPRi-based circuits to control gene expression in plants

MA Khan, G Herring, JY Zhu, M Oliva, E Fourie… - Nature …, 2024 - nature.com
The construction of synthetic gene circuits in plants has been limited by a lack of orthogonal
and modular parts. Here, we implement a CRISPR (clustered regularly interspaced short …

Optogenetic control of gene expression in plants in the presence of ambient white light

R Ochoa-Fernandez, NB Abel, FG Wieland… - Nature …, 2020 - nature.com
Optogenetics is the genetic approach for controlling cellular processes with light. It provides
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …

Rational design of minimal synthetic promoters for plants

YM Cai, K Kallam, H Tidd, G Gendarini… - Nucleic Acids …, 2020 - academic.oup.com
Promoters serve a critical role in establishing baseline transcriptional capacity through the
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …

COP1 destabilizes DELLA proteins in Arabidopsis

N Blanco-Touriñán, M Legris, EG Minguet… - Proceedings of the …, 2020 - pnas.org
DELLA transcriptional regulators are central components in the control of plant growth
responses to the environment. This control is considered to be mediated by changes in the …

Assessment of Cas12a‐mediated gene editing efficiency in plants

JM Bernabé‐Orts, I Casas‐Rodrigo… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering.
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …

Plant synthetic biology innovations for biofuels and bioproducts

Y Yang, TA Chaffin, AH Ahkami, E Blumwald… - Trends in …, 2022 - cell.com
Plant-based biosynthesis of fuels, chemicals, and materials promotes environmental
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …

Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery

A Jozwiak, PD Sonawane, S Panda… - Nature Chemical …, 2020 - nature.com
Glycosylation is one of the most prevalent molecular modifications in nature. Single or
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …

Nimble cloning: a simple, versatile, and efficient system for standardized molecular cloning

P Yan, Y Zeng, W Shen, D Tuo, X Li… - … in bioengineering and …, 2020 - frontiersin.org
Molecular cloning is one of the most fundamental technologies in molecular biology, and
has been critical for driving biotechnological advances. In this study, we have developed a …