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A user's guide to golden gate cloning methods and standards
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …
of applications has driven the development of many different cloning strategies. One method …
Making small molecules in plants: a chassis for synthetic biology‐based production of plant natural products
X Liu, P Zhang, Q Zhao… - Journal of Integrative Plant …, 2023 - Wiley Online Library
Plant natural products have been extensively exploited in food, medicine, flavor, cosmetic,
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …
CRISPRi-based circuits to control gene expression in plants
The construction of synthetic gene circuits in plants has been limited by a lack of orthogonal
and modular parts. Here, we implement a CRISPR (clustered regularly interspaced short …
and modular parts. Here, we implement a CRISPR (clustered regularly interspaced short …
Optogenetic control of gene expression in plants in the presence of ambient white light
Optogenetics is the genetic approach for controlling cellular processes with light. It provides
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …
Rational design of minimal synthetic promoters for plants
YM Cai, K Kallam, H Tidd, G Gendarini… - Nucleic Acids …, 2020 - academic.oup.com
Promoters serve a critical role in establishing baseline transcriptional capacity through the
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …
COP1 destabilizes DELLA proteins in Arabidopsis
DELLA transcriptional regulators are central components in the control of plant growth
responses to the environment. This control is considered to be mediated by changes in the …
responses to the environment. This control is considered to be mediated by changes in the …
Assessment of Cas12a‐mediated gene editing efficiency in plants
JM Bernabé‐Orts, I Casas‐Rodrigo… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering.
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …
Plant synthetic biology innovations for biofuels and bioproducts
Plant-based biosynthesis of fuels, chemicals, and materials promotes environmental
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery
Glycosylation is one of the most prevalent molecular modifications in nature. Single or
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …
Nimble cloning: a simple, versatile, and efficient system for standardized molecular cloning
P Yan, Y Zeng, W Shen, D Tuo, X Li… - … in bioengineering and …, 2020 - frontiersin.org
Molecular cloning is one of the most fundamental technologies in molecular biology, and
has been critical for driving biotechnological advances. In this study, we have developed a …
has been critical for driving biotechnological advances. In this study, we have developed a …