As part of its remit to provide computational support to the cryo-EM community, the
Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM) has produced …

1. Introduction 3082. Electron microscopy 3112.1 Specimen preparation 3112.2 The
electron microscope 3112.3 Acceleration voltage, defocus, and the electron gun 3122.4 …

We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …

We have developed new open-source software called cis TEM (computational imaging
system for transmission electron microscopy) for the processing of data for high-resolution …

Electron cryomicroscopy can, in principle, determine the structures of most biological
molecules but is currently limited by access, specimen preparation difficulties, and cost. We …

We describe a new implementation for the reconstruction of helical assemblies in the
empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for …

Abstract Three-dimensional (3D) structure determination by single particle electron
cryomicroscopy (cryoEM) involves the calculation of an initial 3D model, followed by …

New developments in the program package ATSAS (version 2.4) for the processing and
analysis of isotropic small-angle X-ray and neutron scattering data are described. They …

EMAN is a scientific image processing package with a particular focus on single particle
reconstruction from transmission electron microscopy (TEM) images. It was first released in …

A computational procedure is described for assigning the absolute hand of the structure of a
protein or assembly determined by single-particle electron microscopy. The procedure …