A guide to choosing fluorescent proteins
NC Shaner, PA Steinbach, RY Tsien - Nature methods, 2005 - nature.com
The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,,
promises a wide variety of new tools for biological imaging. With no unified standard for …
promises a wide variety of new tools for biological imaging. With no unified standard for …
Fluorescent proteins and their applications in imaging living cells and tissues
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
A highly photostable and bright green fluorescent protein
M Hirano, R Ando, S Shimozono, M Sugiyama… - Nature …, 2022 - nature.com
The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Fluorescent proteins are genetically encoded, easily imaged reporters crucial in biology and
biotechnology,. When a protein is tagged by fusion to a fluorescent protein, interactions …
biotechnology,. When a protein is tagged by fusion to a fluorescent protein, interactions …
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
A Sakaue-Sawano, H Kurokawa, T Morimura, A Hanyu… - Cell, 2008 - cell.com
The cell-cycle transition from G 1 to S phase has been difficult to visualize. We have
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Detailed analysis of neuronal network architecture requires the development of new
methods. Here we present strategies to visualize synaptic circuits by genetically labelling …
methods. Here we present strategies to visualize synaptic circuits by genetically labelling …
Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
Quantitative assessment of fluorescent proteins
The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …
The fluorescent protein palette: tools for cellular imaging
RN Day, MW Davidson - Chemical Society Reviews, 2009 - pubs.rsc.org
This critical review provides an overview of the continually expanding family of fluorescent
proteins (FPs) that have become essential tools for studies of cell biology and physiology …
proteins (FPs) that have become essential tools for studies of cell biology and physiology …
Improving the photostability of bright monomeric orange and red fluorescent proteins
All organic fluorophores undergo irreversible photobleaching during prolonged illumination.
Although fluorescent proteins typically bleach at a substantially slower rate than many small …
Although fluorescent proteins typically bleach at a substantially slower rate than many small …