A guide to choosing fluorescent proteins

NC Shaner, PA Steinbach, RY Tsien - Nature methods, 2005 - nature.com
The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,,
promises a wide variety of new tools for biological imaging. With no unified standard for …

Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

A highly photostable and bright green fluorescent protein

M Hirano, R Ando, S Shimozono, M Sugiyama… - Nature …, 2022 - nature.com
The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

NC Shaner, RE Campbell, PA Steinbach… - Nature …, 2004 - nature.com
Fluorescent proteins are genetically encoded, easily imaged reporters crucial in biology and
biotechnology,. When a protein is tagged by fusion to a fluorescent protein, interactions …

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression

A Sakaue-Sawano, H Kurokawa, T Morimura, A Hanyu… - Cell, 2008 - cell.com
The cell-cycle transition from G 1 to S phase has been difficult to visualize. We have
harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop …

Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

J Livet, TA Weissman, H Kang, RW Draft, J Lu… - Nature, 2007 - nature.com
Detailed analysis of neuronal network architecture requires the development of new
methods. Here we present strategies to visualize synaptic circuits by genetically labelling …

Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks

EC Greenwald, S Mehta, J Zhang - Chemical reviews, 2018 - ACS Publications
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Quantitative assessment of fluorescent proteins

PJ Cranfill, BR Sell, MA Baird, JR Allen, Z Lavagnino… - Nature …, 2016 - nature.com
The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …

The fluorescent protein palette: tools for cellular imaging

RN Day, MW Davidson - Chemical Society Reviews, 2009 - pubs.rsc.org
This critical review provides an overview of the continually expanding family of fluorescent
proteins (FPs) that have become essential tools for studies of cell biology and physiology …

Improving the photostability of bright monomeric orange and red fluorescent proteins

NC Shaner, MZ Lin, MR McKeown, PA Steinbach… - Nature …, 2008 - nature.com
All organic fluorophores undergo irreversible photobleaching during prolonged illumination.
Although fluorescent proteins typically bleach at a substantially slower rate than many small …