Heterologous protein production using the Pichia pastoris expression system
S Macauley‐Patrick, ML Fazenda, B McNeil… - Yeast, 2005 - Wiley Online Library
The Pichia pastoris expression system is being used successfully for the production of
various recombinant heterologous proteins. Recent developments with respect to the Pichia …
various recombinant heterologous proteins. Recent developments with respect to the Pichia …
Advances in recombinant antibody manufacturing
R Kunert, D Reinhart - Applied microbiology and biotechnology, 2016 - Springer
Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression,
production processes have steadily improved through numerous advances. In this review …
production processes have steadily improved through numerous advances. In this review …
Expression of Recombinant Proteins in Pichia Pastoris
P Li, A Anumanthan, XG Gao, K Ilangovan… - Applied biochemistry …, 2007 - Springer
Pichia pastoris has been used extensively and successfully to express recombinant
proteins. In this review, we summarize the elements required for expressing heterologous …
proteins. In this review, we summarize the elements required for expressing heterologous …
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Different species of microorganisms including yeasts, filamentous fungi and bacteria have
been used in the past 25 years for the controlled production of foreign proteins of scientific …
been used in the past 25 years for the controlled production of foreign proteins of scientific …
Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: a review
Pichia pastoris is currently one of the most effective and versatile systems for the expression
of heterologous proteins. Its success is due to its powerful methanol-inducible alcohol …
of heterologous proteins. Its success is due to its powerful methanol-inducible alcohol …
Stress in recombinant protein producing yeasts
It is well established today that heterologous overexpression of proteins is connected with
different stress reactions. The expression of a foreign protein at a high level may either …
different stress reactions. The expression of a foreign protein at a high level may either …
The Effect of Temperature on the Proteome of Recombinant Pichia pastoris
The impact of environmental factors on the productivity of yeast cells is poorly investigated
so far. Therefore, it is a major concern to improve the understanding of cellular physiology of …
so far. Therefore, it is a major concern to improve the understanding of cellular physiology of …
Enhanced recombinant protein production under special environmental stress
X Chen, C Li, H Liu - Frontiers in Microbiology, 2021 - frontiersin.org
Regardless of bacteria or eukaryotic microorganism hosts, improving their ability to express
heterologous proteins is always a goal worthy of elaborate study. In addition to traditional …
heterologous proteins is always a goal worthy of elaborate study. In addition to traditional …
Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
MA Nieto-Taype, X Garcia-Ortega, J Albiol… - … in bioengineering and …, 2020 - frontiersin.org
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is currently considered one
of the most promising hosts for recombinant protein production (RPP) and metabolites due …
of the most promising hosts for recombinant protein production (RPP) and metabolites due …
An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris
LM Damasceno, I Pla, HJ Chang, L Cohen… - Protein expression and …, 2004 - Elsevier
The expression of a humanized single-chain variable domain fragment antibody (A33scFv)
was optimized for Pichia pastoris with yields exceeding 4gL− 1. A33scFv recognizes a cell …
was optimized for Pichia pastoris with yields exceeding 4gL− 1. A33scFv recognizes a cell …